6QGW

Crystal structure of E.coli BamA beta-barrel in complex with nanobody E6

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Outer membrane protein assembly factor BamA	polymer	411	46022.8	1	UniProt (P0A942) Pfam (PF01103) UniProt (by SIFTS) (P0A940)	Escherichia coli O157:H7
2	B (B)	NanoE6	polymer	123	13402.0	1		Lama glama
3	C, D, E, F, G... (A)	(HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE	non-polymer		306.4	15	Chemie (C8E)
4	R, S (A, B)	water	water		18.0	289	Chemie (HOH)

Sequence modifications

A: 421 - 810 (UniProt: P0A942)

PDB	External Database	Details
Met 400	-	initiating methionine
Gly 401	-	expression tag
Ser 402	-	expression tag
Ser 403	-	expression tag
His 404	-	expression tag
His 405	-	expression tag
His 406	-	expression tag
His 407	-	expression tag
His 408	-	expression tag
His 409	-	expression tag
Ser 410	-	expression tag
Ser 411	-	expression tag
Gly 412	-	expression tag
Glu 413	-	expression tag
Asn 414	-	expression tag
Leu 415	-	expression tag
Tyr 416	-	expression tag
Phe 417	-	expression tag
Gln 418	-	expression tag
His 419	-	expression tag
Met 420	-	expression tag
Ser 690	Cys 690	conflict
Ser 700	Cys 700	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59424.8
Non-Polymers*	Number of molecules	15
	Total formula weight	4596.6
All*	Total formula weight	64021.3

*Water molecules are not included.