6QGW
Crystal structure of E.coli BamA beta-barrel in complex with nanobody E6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99998 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 56.406, 135.542, 89.754 |
| Unit cell angles | 90.00, 102.92, 90.00 |
Refinement procedure
| Resolution | 43.741 - 1.938 |
| R-factor | 0.1911 |
| Rwork | 0.189 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n75 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.741 | 1.980 |
| High resolution limit [Å] | 1.938 | 1.938 |
| Rmerge | 0.116 | 0.996 |
| Rpim | 0.081 | 0.673 |
| Number of reflections | 47131 | 3001 |
| <I/σ(I)> | 6.6 | 1.2 |
| Completeness [%] | 97.1 | 92.6 |
| Redundancy | 5.9 | 6 |
| CC(1/2) | 0.995 | 0.556 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 25% PEG 4000, 0.2 M MgCl2, 0.1 M Tris pH 8.5 |
