6QGQ

Crystal structure of APT1 C2S mutant bound to palmitic acid.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, D (A, C, D)	Acyl-protein thioesterase 1	polymer	233	24977.8	3	UniProt (O75608) Pfam (PF02230)	Homo sapiens (Human)	hAPT1,Lysophospholipase 1,Lysophospholipase I,LysoPLA I
2	B (B)	Acyl-protein thioesterase 1	polymer	234	25048.9	1	UniProt (O75608) Pfam (PF02230)	Homo sapiens (Human)	hAPT1,Lysophospholipase 1,Lysophospholipase I,LysoPLA I
3	E, F, H, I (A, C, D)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
4	G, J (B, D)	PALMITIC ACID	non-polymer		256.4	2	Chemie (PLM)
5	K, L, M, N (A, B, C, D)	water	water		18.0	132	Chemie (HOH)

Sequence modifications

A, C, D: 1 - 230 (UniProt: O75608)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
Arg 0	-	expression tag
Ser 2	Cys 2	engineered mutation

B: 1 - 230 (UniProt: O75608)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
Arg 0	-	expression tag
Ser 2	Cys 2	engineered mutation
Ala 231	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	99982.3
Non-Polymers*	Number of molecules	6
	Total formula weight	881.2
All*	Total formula weight	100863.5

*Water molecules are not included.