6QDX

Structure of E.coli RlmJ in complex with a bisubstrate analogue (BA4)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Ribosomal RNA large subunit methyltransferase J	polymer	283	32272.0	4	UniProt (A0A0G3KF30) Pfam (PF04378) UniProt (by SIFTS) (P37634) In PDB	Escherichia coli	23STSA4, rRNA (adenine(2030)-N6)-methyltransferase,23S rRNA m6A2030 methyltransferase
2	A, B, C, D	(2~{S})-4-[[(2~{S},3~{S},4~{R},5~{R})-5-(6-aminopurin-9-yl)-3,4-bis(oxidanyl)oxolan-2-yl]methyl-[3-[[9-[(2~{S},3~{R},4~{S},5~{S})-5-(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl]purin-6-yl]amino]propyl]amino]-2-azanyl-butanoic acid	non-polymer		674.7	4	Chemie (HY8)
3		water	water		18.0	711	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 280 (UniProt: A0A0G3KF30)

PDB	External Database	Details
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Lys 60	Glu 60	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	129087.8
Non-Polymers*	Number of molecules	4
	Total formula weight	2698.7
All*	Total formula weight	131786.5

*Water molecules are not included.