6QDX

Structure of E.coli RlmJ in complex with a bisubstrate analogue (BA4)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003723	molecular_function	RNA binding
A	0005515	molecular_function	protein binding
A	0005829	cellular_component	cytosol
A	0006364	biological_process	rRNA processing
A	0008168	molecular_function	methyltransferase activity
A	0008649	molecular_function	rRNA methyltransferase activity
A	0008988	molecular_function	rRNA (adenine-N6-)-methyltransferase activity
A	0015976	biological_process	carbon utilization
A	0016740	molecular_function	transferase activity
A	0032259	biological_process	methylation
A	0036307	molecular_function	23S rRNA (adenine(2030)-N(6))-methyltransferase activity
A	0043412	biological_process	macromolecule modification
A	0070475	biological_process	rRNA base methylation
B	0003676	molecular_function	nucleic acid binding
B	0003723	molecular_function	RNA binding
B	0005515	molecular_function	protein binding
B	0005829	cellular_component	cytosol
B	0006364	biological_process	rRNA processing
B	0008168	molecular_function	methyltransferase activity
B	0008649	molecular_function	rRNA methyltransferase activity
B	0008988	molecular_function	rRNA (adenine-N6-)-methyltransferase activity
B	0015976	biological_process	carbon utilization
B	0016740	molecular_function	transferase activity
B	0032259	biological_process	methylation
B	0036307	molecular_function	23S rRNA (adenine(2030)-N(6))-methyltransferase activity
B	0043412	biological_process	macromolecule modification
B	0070475	biological_process	rRNA base methylation
C	0003676	molecular_function	nucleic acid binding
C	0003723	molecular_function	RNA binding
C	0005515	molecular_function	protein binding
C	0005829	cellular_component	cytosol
C	0006364	biological_process	rRNA processing
C	0008168	molecular_function	methyltransferase activity
C	0008649	molecular_function	rRNA methyltransferase activity
C	0008988	molecular_function	rRNA (adenine-N6-)-methyltransferase activity
C	0015976	biological_process	carbon utilization
C	0016740	molecular_function	transferase activity
C	0032259	biological_process	methylation
C	0036307	molecular_function	23S rRNA (adenine(2030)-N(6))-methyltransferase activity
C	0043412	biological_process	macromolecule modification
C	0070475	biological_process	rRNA base methylation
D	0003676	molecular_function	nucleic acid binding
D	0003723	molecular_function	RNA binding
D	0005515	molecular_function	protein binding
D	0005829	cellular_component	cytosol
D	0006364	biological_process	rRNA processing
D	0008168	molecular_function	methyltransferase activity
D	0008649	molecular_function	rRNA methyltransferase activity
D	0008988	molecular_function	rRNA (adenine-N6-)-methyltransferase activity
D	0015976	biological_process	carbon utilization
D	0016740	molecular_function	transferase activity
D	0032259	biological_process	methylation
D	0036307	molecular_function	23S rRNA (adenine(2030)-N(6))-methyltransferase activity
D	0043412	biological_process	macromolecule modification
D	0070475	biological_process	rRNA base methylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	23
Details	binding site for residue HY8 A 301

Chain	Residue
A	HIS9
A	GLY99
A	SER100
A	ASP164
A	PRO166
A	TYR167
A	GLU168
A	MET235
A	HOH429
A	HOH431
A	HOH433
A	ALA10
A	HOH434
A	HOH500
A	HOH541
B	HOH447
A	ASP15
A	LYS18
A	HIS19
A	ASP40
A	HIS42
A	ALA43
A	GLY44

---

site_id	AC2
Number of Residues	24
Details	binding site for residue HY8 B 301

Chain	Residue
B	HIS9
B	ASN12
B	ALA14
B	ASP15
B	LYS18
B	HIS19
B	HIS42
B	ALA43
B	GLY44
B	ALA45
B	TYR48
B	LYS60
B	GLY99
B	SER100
B	HIS120
B	ASP123
B	ASP164
B	PRO165
B	PRO166
B	TRP195
B	HOH422
B	HOH460
B	HOH465
B	HOH502

---

site_id	AC3
Number of Residues	21
Details	binding site for residue HY8 C 301

Chain	Residue
C	HIS9
C	ALA10
C	ASP15
C	LYS18
C	HIS19
C	ASP40
C	HIS42
C	ALA43
C	GLY44
C	GLY99
C	SER100
C	ASP164
C	PRO166
C	GLU168
C	HOH402
C	HOH416
C	HOH423
C	HOH458
C	HOH494
C	HOH499
C	HOH524

---

site_id	AC4
Number of Residues	17
Details	binding site for residue HY8 D 301

Chain	Residue
D	HIS9
D	ALA10
D	ASP15
D	LYS18
D	HIS19
D	HIS42
D	ALA43
D	GLY44
D	GLY99
D	SER100
D	GLU118
D	HIS120
D	ASP123
D	ASP164
D	PRO166
D	HOH407
D	HOH409

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Functional Information from PROSITE/UniProt

site_id	PS00092
Number of Residues	7
Details	N6_MTASE N-6 Adenine-specific DNA methylases signature. ILIDPPY

Chain	Residue	Details
A	ILE161-TYR167
A	ILE241-TRP247

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	4
Details	ACT_SITE: Proton acceptor => ECO:0000305|PubMed:23945937

Chain	Residue	Details
A	ASP164
B	ASP164
C	ASP164
D	ASP164

---

site_id	SWS_FT_FI2
Number of Residues	24
Details	BINDING: BINDING => ECO:0000305|PubMed:23945937

Chain	Residue	Details
A	HIS19
B	GLU118
B	ASP143
B	ASP164
C	HIS19
C	HIS42
C	SER100
C	GLU118
C	ASP143
C	ASP164
D	HIS19
A	HIS42
D	HIS42
D	SER100
D	GLU118
D	ASP143
D	ASP164
A	SER100
A	GLU118
A	ASP143
A	ASP164
B	HIS19
B	HIS42
B	SER100

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	SITE: Interaction with substrate rRNA => ECO:0000305

Chain	Residue	Details
A	TYR4
B	TYR4
C	TYR4
D	TYR4

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