6Q4T
KOD DNA pol in a closed ternary complex with 7-deaza-7-(2-(2-hydroxyethoxy)-N-(prop-2-yn-1-yl)acetamide)-2-dATP
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | DNA polymerase,DNA polymerase,DNA polymerase | polymer | 774 | 90060.5 | 1 | UniProt (P77933) Pfam (PF03104) Pfam (PF00136) In PDB | Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1)) | |
2 | T | DNA (5'-D(P*AP*AP*CP*TP*GP*TP*GP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3') | polymer | 16 | 4930.2 | 1 | synthetic construct | ||
3 | P | DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*CP*AP*C)-3') | polymer | 12 | 3601.4 | 1 | synthetic construct | ||
4 | A | MANGANESE (II) ION | non-polymer | 54.9 | 1 | Chemie (MN) | |||
5 | A, T | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
6 | A | 1,2-ETHANEDIOL | non-polymer | 62.1 | 7 | Chemie (EDO) | |||
7 | A | [[(2~{R},3~{S},5~{R})-5-[4-azanyl-5-[3-[2-(2-hydroxyethyloxy)ethanoylamino]prop-1-ynyl]pyrrolo[2,3-d]pyrimidin-7-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate | non-polymer | 645.3 | 1 | Chemie (HHZ) | |||
8 | A | NITRATE ION | non-polymer | 62.0 | 2 | Chemie (NO3) | |||
9 | A | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
10 | water | water | 18.0 | 277 | Chemie (HOH) |
Sequence modifications
A: 1 - 406 (UniProt: P77933)
A: 407 - 491 (UniProt: P77933)
A: 492 - 774 (UniProt: P77933)
PDB | External Database | Details |
---|---|---|
Ala 141 | Asp 141 | engineered mutation |
Ala 143 | Glu 143 | engineered mutation |
A: 492 - 774 (UniProt: P77933)
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 98592.0 | |
Non-Polymers* | Number of molecules | 14 |
Total formula weight | 1403.4 | |
All* | Total formula weight | 99995.5 |