6Q4T

KOD DNA pol in a closed ternary complex with 7-deaza-7-(2-(2-hydroxyethoxy)-N-(prop-2-yn-1-yl)acetamide)-2-dATP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA polymerase,DNA polymerase,DNA polymerase	polymer	774	90060.5	1	UniProt (P77933) Pfam (PF03104) Pfam (PF00136)	Thermococcus kodakarensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Pyrococcus kodakaraensis (strain KOD1))
2	B (T)	DNA (5'-D(P*AP*AP*CP*TP*GP*TP*GP*GP*CP*CP*GP*TP*GP*GP*TP*C)-3')	polymer	16	4930.2	1		synthetic construct
3	C (P)	DNA (5'-D(*GP*AP*CP*CP*AP*CP*GP*GP*CP*CP*AP*C)-3')	polymer	12	3601.4	1		synthetic construct
4	D (A)	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
5	E, Q (A, T)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	F, G, H, I, J... (A)	1,2-ETHANEDIOL	non-polymer		62.1	7	Chemie (EDO)
7	M (A)	[[(2~{R},3~{S},5~{R})-5-[4-azanyl-5-[3-[2-(2-hydroxyethyloxy)ethanoylamino]prop-1-ynyl]pyrrolo[2,3-d]pyrimidin-7-yl]-3-oxidanyl-oxolan-2-yl]methoxy-oxidanyl-phosphoryl] phosphono hydrogen phosphate	non-polymer		645.3	1	Chemie (HHZ)
8	N, O (A)	NITRATE ION	non-polymer		62.0	2	Chemie (NO3)
9	P (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
10	R, S, T (A, T, P)	water	water		18.0	277	Chemie (HOH)

Sequence modifications

A: 1 - 406 (UniProt: P77933)

PDB	External Database	Details
Ala 141	Asp 141	engineered mutation
Ala 143	Glu 143	engineered mutation

A: 407 - 491 (UniProt: P77933)
A: 492 - 774 (UniProt: P77933)

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	98592.0
Non-Polymers*	Number of molecules	14
	Total formula weight	1403.4
All*	Total formula weight	99995.5

*Water molecules are not included.