6Q2N

Cryo-EM structure of RET/GFRa1/GDNF extracellular complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Glial cell line-derived neurotrophic factor	polymer	134	15096.2	2	UniProt (P39905) Pfam (PF00019) In PDB	Homo sapiens (Human)	hGDNF,Astrocyte-derived trophic factor,ATF
2	C, D	GDNF family receptor alpha-1	polymer	412	46358.6	2	UniProt (P56159) Pfam (PF02351) In PDB	Homo sapiens (Human)	GFR-alpha-1,RET ligand 1,TGF-beta-related neurotrophic factor receptor 1
3	E, F	Proto-oncogene tyrosine-protein kinase receptor Ret	polymer	617	69100.8	2	UniProt (P07949) Pfam (PF17756) Pfam (PF00028) Pfam (PF17812) Pfam (PF17813) Pfam (PF22540) In PDB	Homo sapiens (Human)	Cadherin family member 12,Proto-oncogene c-Ret
4	E, F	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)

Sequence modifications

C, D: 25 - 426 (UniProt: P56159)

PDB	External Database	Details
Gly 427	-	expression tag
Thr 428	-	expression tag
His 429	-	expression tag
His 430	-	expression tag
His 431	-	expression tag
His 432	-	expression tag
His 433	-	expression tag
His 434	-	expression tag
His 435	-	expression tag
His 436	-	expression tag

E, F: 29 - 635 (UniProt: P07949)

PDB	External Database	Details
His 114	Arg 114	conflict
Gly 636	-	expression tag
Thr 637	-	expression tag
His 638	-	expression tag
His 639	-	expression tag
His 640	-	expression tag
His 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag
His 644	-	expression tag
His 645	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	261111.2
Non-Polymers*	Number of molecules	8
	Total formula weight	320.6
All*	Total formula weight	261431.8

*Water molecules are not included.