6Q2J

Cryo-EM structure of extracellular dimeric complex of RET/GFRAL/GDF15

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D (A, B)	Growth/differentiation factor 15	polymer	135	14879.1	2	UniProt (Q99988) Pfam (PF00019)	Homo sapiens (Human)	GDF-15,Macrophage inhibitory cytokine 1,MIC-1,NSAID-activated gene 1 protein,NAG-1,NSAID-regulated gene 1 protein,NRG-1,Placental TGF-beta,Placental bone morphogenetic protein,Prostate differentiation factor
2	B, E (C, D)	GDNF family receptor alpha-like	polymer	343	39120.6	2	UniProt (Q6UXV0) Pfam (PF02351)	Homo sapiens (Human)
3	C, F (E, F)	Proto-oncogene tyrosine-protein kinase receptor Ret	polymer	617	69100.8	2	UniProt (P07949) Pfam (PF17756) Pfam (PF00028) Pfam (PF17812) Pfam (PF17813) Pfam (PF22540)	Homo sapiens (Human)	Cadherin family member 12,Proto-oncogene c-Ret
4	G, H, I, J, K... (E, F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	14	Chemie (NAG)
5	AA, BA, N, O, P... (F, E)	CALCIUM ION	non-polymer		40.1	8	Chemie (CA)

Sequence modifications

A, B: 197 - 308 (UniProt: Q99988)

PDB	External Database	Details
Met 174	-	initiating methionine
Gly 175	-	expression tag
Ser 176	-	expression tag
Ser 177	-	expression tag
His 178	-	expression tag
His 179	-	expression tag
His 180	-	expression tag
His 181	-	expression tag
His 182	-	expression tag
His 183	-	expression tag
Ser 184	-	expression tag
Ser 185	-	expression tag
Gly 186	-	expression tag
Leu 187	-	expression tag
Glu 188	-	expression tag
Val 189	-	expression tag
Leu 190	-	expression tag
Phe 191	-	expression tag
Gln 192	-	expression tag
Gly 193	-	expression tag
Pro 194	-	expression tag
His 195	-	expression tag
Met 196	-	expression tag

C, D: 20 - 352 (UniProt: Q6UXV0)

PDB	External Database	Details
Gly 353	-	expression tag
Thr 354	-	expression tag
His 355	-	expression tag
His 356	-	expression tag
His 357	-	expression tag
His 358	-	expression tag
His 359	-	expression tag
His 360	-	expression tag
His 361	-	expression tag
His 362	-	expression tag

E, F: 29 - 635 (UniProt: P07949)

PDB	External Database	Details
His 114	Arg 114	conflict
Gly 636	-	expression tag
Thr 637	-	expression tag
His 638	-	expression tag
His 639	-	expression tag
His 640	-	expression tag
His 641	-	expression tag
His 642	-	expression tag
His 643	-	expression tag
His 644	-	expression tag
His 645	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	246201.0
Non-Polymers*	Number of molecules	22
	Total formula weight	3417.5
All*	Total formula weight	249618.6

*Water molecules are not included.