6Q0V

Structure of DDB1-DDA1-DCAF15 complex bound to tasisulam and RBM39

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA damage-binding protein 1	polymer	864	96425.6	1	UniProt (Q16531) Pfam (PF10433) Pfam (PF03178)	Homo sapiens (Human)	DDB p127 subunit,DNA damage-binding protein a,DDBa,Damage-specific DNA-binding protein 1,HBV X-associated protein 1,XAP-1,UV-damaged DNA-binding factor,UV-damaged DNA-binding protein 1,UV-DDB 1,XPE-binding factor,XPE-BF,Xeroderma pigmentosum group E-complementing protein,XPCe
2	B (B)	DDB1- and CUL4-associated factor 15	polymer	276	31611.0	1	UniProt (Q66K64) Pfam (PF14939)	Homo sapiens (Human)
3	C (C)	DDB1- and CUL4-associated factor 15	polymer	263	29893.5	1	UniProt (Q66K64)	Homo sapiens (Human)
4	D (D)	RNA-binding protein 39	polymer	107	12062.6	1	UniProt (Q14498) Pfam (PF00076)	Homo sapiens (Human)	CAPER alpha,Hepatocellular carcinoma protein 1,RNA-binding motif protein 39,RNA-binding region-containing protein 2,Splicing factor HCC1
5	E (E)	DET1- and DDB1-associated protein 1	polymer	126	14542.2	1	UniProt (Q9BW61) Pfam (PF10172)	Homo sapiens (Human)	Placenta cross-immune reaction antigen 1,PCIA-1
6	F (B)	N-[(5-bromothiophen-2-yl)sulfonyl]-2,4-dichlorobenzamide	non-polymer		415.1	1	Chemie (P7M)
7	G (B)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
8	H, I, J, K, L (A, B, C, D, E)	water	water		18.0	17	Chemie (HOH)

Sequence modifications

A: 1 - 699 (UniProt: Q16531)

PDB	External Database	Details
Met -27	-	initiating methionine
Gly -26	-	expression tag
Ser -25	-	expression tag
Ser -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Ser -17	-	expression tag
Ala -16	-	expression tag
Ala -15	-	expression tag
His -14	-	expression tag
Ile -13	-	expression tag
Val -12	-	expression tag
Met -11	-	expression tag
Val -10	-	expression tag
Asp -9	-	expression tag
Ala -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Pro -5	-	expression tag
Thr -4	-	expression tag
Lys -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

A: 706 - 1140 (UniProt: Q16531)

PDB	External Database	Details
Gly 700	-	linker
Asn 701	-	linker
Gly 702	-	linker
Asn 703	-	linker
Ser 704	-	linker
Gly 705	-	linker

B: 34 - 260 (UniProt: Q66K64)

PDB	External Database	Details
Met -15	-	initiating methionine
Asp -14	-	expression tag
Trp -13	-	expression tag
Ser -12	-	expression tag
His -11	-	expression tag
Pro -10	-	expression tag
Gln -9	-	expression tag
Phe -8	-	expression tag
Glu -7	-	expression tag
Lys -6	-	expression tag
Ser -5	-	expression tag
Ala -4	-	expression tag
Val -3	-	expression tag
Gly -2	-	expression tag
Leu -1	-	expression tag
Asn 0	-	expression tag
Asp 1	-	expression tag
Ile 2	-	expression tag
Phe 3	-	expression tag
Glu 4	-	expression tag
Ala 5	-	expression tag
Gln 6	-	expression tag
Lys 7	-	expression tag
Ile 8	-	expression tag
Glu 9	-	expression tag
Trp 10	-	expression tag
His 11	-	expression tag
Glu 12	-	expression tag
Gly 13	-	expression tag
Gly 14	-	expression tag
Gly 15	-	expression tag
Gly 16	-	expression tag
Ser 17	-	expression tag
Gly 18	-	expression tag
Glu 19	-	expression tag
Asn 20	-	expression tag
Leu 21	-	expression tag
Tyr 22	-	expression tag
Phe 23	-	expression tag
Gln 24	-	expression tag
Gly 25	-	expression tag
Gly 26	-	expression tag
Gly 27	-	expression tag
Arg 28	-	expression tag
Met 29	-	expression tag
Gly 30	-	expression tag
Arg 31	-	expression tag
Arg 32	-	expression tag
Arg 33	-	expression tag

C: 383 - 600 (UniProt: Q66K64)

PDB	External Database	Details
Met 338	-	initiating methionine
Asp 339	-	expression tag
Trp 340	-	expression tag
Ser 341	-	expression tag
His 342	-	expression tag
Pro 343	-	expression tag
Gln 344	-	expression tag
Phe 345	-	expression tag
Glu 346	-	expression tag
Lys 347	-	expression tag
Ser 348	-	expression tag
Ala 349	-	expression tag
Val 350	-	expression tag
Gly 351	-	expression tag
Leu 352	-	expression tag
Asn 353	-	expression tag
Asp 354	-	expression tag
Ile 355	-	expression tag
Phe 356	-	expression tag
Glu 357	-	expression tag
Ala 358	-	expression tag
Gln 359	-	expression tag
Lys 360	-	expression tag
Ile 361	-	expression tag
Glu 362	-	expression tag
Trp 363	-	expression tag
His 364	-	expression tag
Glu 365	-	expression tag
Gly 366	-	expression tag
Gly 367	-	expression tag
Gly 368	-	expression tag
Gly 369	-	expression tag
Ser 370	-	expression tag
Gly 371	-	expression tag
Glu 372	-	expression tag
Asn 373	-	expression tag
Leu 374	-	expression tag
Tyr 375	-	expression tag
Phe 376	-	expression tag
Gln 377	-	expression tag
Gly 378	-	expression tag
Gly 379	-	expression tag
Gly 380	-	expression tag
Arg 381	-	expression tag
Met 382	-	expression tag

D: 250 - 332 (UniProt: Q14498)

PDB	External Database	Details
Met 226	-	initiating methionine
Gly 227	-	expression tag
Ser 228	-	expression tag
Ser 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
Ser 236	-	expression tag
Ala 237	-	expression tag
Val 238	-	expression tag
Asp 239	-	expression tag
Glu 240	-	expression tag
Asn 241	-	expression tag
Leu 242	-	expression tag
Tyr 243	-	expression tag
Phe 244	-	expression tag
Gln 245	-	expression tag
Gly 246	-	expression tag
Gly 247	-	expression tag
Gly 248	-	expression tag
Arg 249	-	expression tag

E: 1 - 102 (UniProt: Q9BW61)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
Ser -21	-	expression tag
Ser -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
Ser -13	-	expression tag
Ala -12	-	expression tag
Val -11	-	expression tag
Asp -10	-	expression tag
Glu -9	-	expression tag
Asn -8	-	expression tag
Leu -7	-	expression tag
Tyr -6	-	expression tag
Phe -5	-	expression tag
Gln -4	-	expression tag
Gly -3	-	expression tag
Gly -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	5
	Total formula weight	184534.9
Non-Polymers*	Number of molecules	2
	Total formula weight	480.5
All*	Total formula weight	185015.4

*Water molecules are not included.