6Q0V
Structure of DDB1-DDA1-DCAF15 complex bound to tasisulam and RBM39
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.6531 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.769, 94.613, 260.390 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.550 - 2.900 |
R-factor | 0.207 |
Rwork | 0.206 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6q0r |
Data reduction software | XDS (20190315) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.2) |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.310 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.217 | 5.368 |
Number of reflections | 45408 | 4298 |
<I/σ(I)> | 15.3 | |
Completeness [%] | 99.7 | |
Redundancy | 39.1 | |
CC(1/2) | 0.990 | 0.770 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 300 | 20% PEG 4000 |