6PWW
Cryo-EM structure of MLL1 in complex with RbBP5 and WDR5 bound to the nucleosome
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Retinoblastoma-binding protein 5 | polymer | 538 | 59179.4 | 1 | UniProt (Q15291) Pfam (PF00400) | Homo sapiens (Human) | RBBP-5,Retinoblastoma-binding protein RBQ-3 |
| 2 | B (B) | WD repeat-containing protein 5 | polymer | 313 | 34391.0 | 1 | UniProt (P61964) Pfam (PF25175) | Homo sapiens (Human) | BMP2-induced 3-kb gene protein |
| 3 | C (C) | Histone-lysine N-methyltransferase 2A | polymer | 209 | 24141.7 | 1 | UniProt (Q03164) Pfam (PF00856) | Homo sapiens (Human) | Lysine N-methyltransferase 2A,ALL-1,CXXC-type zinc finger protein 7,Myeloid/lymphoid or mixed-lineage leukemia,Myeloid/lymphoid or mixed-lineage leukemia protein 1,Trithorax-like protein,Zinc finger protein HRX |
| 4 | D, H (G, K) | Histone H3.2 | polymer | 136 | 15435.1 | 2 | UniProt (P84233) Pfam (PF00125) | Xenopus laevis (African clawed frog) | Histone H3 |
| 5 | E, M (H, L) | Histone H4 | polymer | 103 | 11394.4 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 6 | F, I (I, M) | Histone H2A type 1 | polymer | 129 | 13978.2 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 7 | G, J (J, N) | Histone H2B 1.1 | polymer | 123 | 13655.9 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 8 | K (O) | DNA (146-MER) | polymer | 147 | 45138.8 | 1 | synthetic construct | ||
| 9 | L (P) | DNA (146-MER) | polymer | 147 | 45610.0 | 1 | synthetic construct | ||
| 10 | N (C) | S-ADENOSYL-L-HOMOCYSTEINE | non-polymer | 384.4 | 1 | Chemie (SAH) PubChem (193) PubChem (13792) PubChem (189149) PubChem (439155) PubChem (5284331) PubChem (71170554) PubChem (13231890) PubChem (16760024) PubChem (25210495) PubChem (44361184) PubChem (177678424) PubChem (46936206) PubChem (21125603) PubChem (25203782) PubChem (52224400) PubChem (50989525) PubChem (51380914) PubChem (52224398) PubChem (6419189) PubChem (57544532) PubChem (59046843) PubChem (59170628) PubChem (164157219) PubChem (59576228) PubChem (66805708) PubChem (70689219) PubChem (70702054) PubChem (70832431) PubChem (118374891) PubChem (118560068) PubChem (126969526) PubChem (129105892) PubChem (143518505) PubChem (154578335) PubChem (163054214) PubChem (163335525) | |||
| 11 | O (C) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) |
Sequence modifications
A: 2 - 538 (UniProt: Q15291)
C: 3762 - 3969 (UniProt: Q03164)
G, K: 0 - 135 (UniProt: P84233)
I, M: 1 - 129 (UniProt: P06897)
J, N: 1 - 122 (UniProt: P02281)
| PDB | External Database | Details |
|---|---|---|
| Ser 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ser 3761 | - | expression tag |
| Ile 3861 | Asn 3861 | conflict |
| Leu 3867 | Gln 3867 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Ala 102 | Gly 103 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Arg 99 | Gly 100 | conflict |
| Ser 123 | Ala 124 | conflict |
| PDB | External Database | Details |
|---|---|---|
| Met 0 | - | initiating methionine |
| Thr 29 | Ser 33 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 13 |
| Total formula weight | 317388.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 449.8 | |
| All* | Total formula weight | 317838.2 |
