6PCV

Single Particle Reconstruction of Phosphatidylinositol (3,4,5) trisphosphate-dependent Rac exchanger 1 bound to G protein beta gamma subunits

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Phosphatidylinositol (3,4,5) trisphosphate-dependent Rac exchanger 1	polymer	1635	184840.9	1	UniProt (A0A2X0SFH1) Pfam (PF00621) Pfam (PF22697) Pfam (PF00610) UniProt (by SIFTS) (Q8TCU6)	Homo sapiens (Human)	PREX1, RAC EXCHANGER 1
2	B (B)	Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1	polymer	340	37416.9	1	UniProt (P62871) Pfam (PF00400)	Bos taurus (Bovine)	Transducin beta chain 1
3	C (G)	Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2	polymer	81	9226.5	1	UniProt (P63212) Pfam (PF00631)	Bos taurus (Bovine)	G gamma-I

Sequence modifications

A: 38 - 1659 (UniProt: A0A2X0SFH1)

PDB	External Database	Details
Gly -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
His 1660	-	expression tag
His 1661	-	expression tag
His 1662	-	expression tag
His 1663	-	expression tag
His 1664	-	expression tag
His 1665	-	expression tag
His 1666	-	expression tag
His 1667	-	expression tag
His 1668	-	expression tag
His 1669	-	expression tag

G: 1 - 71 (UniProt: P63212)

PDB	External Database	Details
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ser 68	Cys 68	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	231484.4
All*	Total formula weight	231484.4

*Water molecules are not included.