6PC1

Crystal structure of Helicobacter pylori PPX/GppA (E143A) in complex with ppGpp

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Guanosine pentaphosphate phosphohydrolase	polymer	495	55597.5	4	UniProt (B5ZA44) Pfam (PF02541) Pfam (PF21447)	Helicobacter pylori (strain G27)
2	E, K, P, U (A, B, C, D)	GUANOSINE-5',3'-TETRAPHOSPHATE	non-polymer		603.2	4	Chemie (G4P)
3	F, L, Q, T, V (A, B, C, D)	PHOSPHATE ION	non-polymer		95.0	5	Chemie (PO4)
4	G, H, M, R, W (A, B, C, D)	(2S)-2-hydroxybutanedioic acid	non-polymer		134.1	5	Chemie (LMR)
5	I, N (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
6	J, O, S (B, C)	MAGNESIUM ION	non-polymer		24.3	3	Chemie (MG)
7	AA, X, Y, Z (D, A, B, C)	water	water		18.0	369	Chemie (HOH)

Sequence modifications

A, B, C, D: 2 - 484 (UniProt: B5ZA44)

PDB	External Database	Details
Met -10	-	initiating methionine
Arg -9	-	expression tag
Gly -8	-	expression tag
Ser -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Ser 1	-	expression tag
Ala 143	Glu 143	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	222390.0
Non-Polymers*	Number of molecules	19
	Total formula weight	3755.0
All*	Total formula weight	226145.0

*Water molecules are not included.