6PC1
Crystal structure of Helicobacter pylori PPX/GppA (E143A) in complex with ppGpp
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-06 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1 |
| Spacegroup name | I 4 |
| Unit cell lengths | 194.507, 194.507, 136.862 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.760 |
| R-factor | 0.1791 |
| Rwork | 0.178 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hi0 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.586 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3352: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.760 | 2.700 |
| Rpim | 0.085 | |
| Number of reflections | 63095 | 5219 |
| <I/σ(I)> | 7.4 | 0.7 |
| Completeness [%] | 99.3 | 74.6 |
| Redundancy | 4.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (v/v) PEG3350, 0.2 M Ammonium tartrate, 15 mM Malic acid |
