6P09

Human DNA Ligase 1 Bound to an Adenylated, dideoxy Terminated DNA nick with 200 mM Mg2+

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA ligase 1	polymer	645	71786.0	1	UniProt (P18858) Pfam (PF04675) Pfam (PF01068) Pfam (PF04679)	Homo sapiens (Human)	DNA ligase I,Polydeoxyribonucleotide synthase [ATP] 1
2	B (B)	DNA (5'-D(*GP*CP*TP*GP*AP*TP*GP*CP*GP*TP*C)-3')	polymer	11	3365.2	1		synthetic construct
3	C (C)	DNA (5'-D(P*GP*TP*CP*GP*GP*AP*C)-3')	polymer	7	2138.4	1		synthetic construct
4	D (D)	DNA (5'-D(*GP*TP*CP*CP*GP*AP*CP*GP*AP*CP*GP*CP*AP*TP*CP*AP*GP*C)-3')	polymer	18	5486.6	1		synthetic construct
5	E, F, G, H, K (A, C)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG) PubChem (888)
6	I (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG) PubChem (8117)
7	J (C)	ADENOSINE MONOPHOSPHATE	non-polymer		347.2	1	Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (40459109) PubChem (46875232) PubChem (40467049) PubChem (40469468) PubChem (44348067) PubChem (45358029) PubChem (177678969) PubChem (6083) PubChem (46874558) PubChem (1549371) PubChem (92144436) PubChem (57358045) PubChem (12358354) PubChem (57879893) PubChem (57879902) PubChem (224) PubChem (122197642) PubChem (34768) PubChem (83862) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (5316444) PubChem (5742864) PubChem (12163610) PubChem (12358355) PubChem (162290139) PubChem (162946266) PubChem (171699505)
8	L, M, N, O (A, B, C, D)	water	water		18.0	562	Chemie (HOH)

Sequence modifications

A: 262 - 904 (UniProt: P18858)

PDB	External Database	Details
Ser 260	-	expression tag
Asn 261	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	82776.2
Non-Polymers*	Number of molecules	7
	Total formula weight	574.9
All*	Total formula weight	83351.1

*Water molecules are not included.