6P09
Human DNA Ligase 1 Bound to an Adenylated, dideoxy Terminated DNA nick with 200 mM Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.99203 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.039, 101.083, 115.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.020 - 2.052 |
| R-factor | 0.1717 |
| Rwork | 0.170 |
| R-free | 0.20080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xn9 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.804 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 4.420 | 2.050 |
| Rmerge | 0.100 | 0.044 | |
| Rmeas | 0.098 | 0.047 | |
| Rpim | 0.043 | 0.018 | 0.604 |
| Total number of observations | 388082 | ||
| Number of reflections | 53293 | 5631 | 5129 |
| <I/σ(I)> | 7.8 | ||
| Completeness [%] | 99.7 | 99.8 | 97.4 |
| Redundancy | 7.3 | 7 | 6.5 |
| CC(1/2) | 0.999 | 0.510 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 100 mM MES, 100 mM Lithium Acetate, 15% (w/v) polyethylene glycol 3350 |
