6OL9

Structure of the M5 muscarinic acetylcholine receptor (M5-T4L) bound to tiotropium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Muscarinic acetylcholine receptor M5, T4 Lysozyme fusion	polymer	480	55093.6	1	UniProt (P08912) UniProt (D9IEF7) Pfam (PF00001) Pfam (PF00959)	Homo sapiens (Human)	Lysis protein,Lysozyme,Muramidase
2	B, C (A)	OLEIC ACID	non-polymer		282.5	2	Chemie (OLA)
3	D (A)	(1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane	non-polymer		392.5	1	Chemie (0HK)
4	E (A)	3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL	non-polymer		326.4	1	Chemie (P33)
5	F (A)	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	1	Chemie (OLC)
6	G (A)	water	water		18.0	7	Chemie (HOH)

Sequence modifications

A: 21 - 224 (UniProt: P08912)

PDB	External Database	Details
Gly 19	-	expression tag
Ala 20	-	expression tag
Arg 117	Ser 117	conflict

A: 1001 - 1160 (UniProt: D9IEF7)

PDB	External Database	Details
Ala 1096	Cys 97	conflict

A: 431 - 532 (UniProt: P08912)

PDB	External Database	Details
Ser 533	-	expression tag
Ser 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag
His 538	-	expression tag
His 539	-	expression tag
His 540	-	expression tag
His 541	-	expression tag
His 542	-	expression tag
His 543	-	expression tag
His 544	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	55093.6
Non-Polymers*	Number of molecules	5
	Total formula weight	1640.4
All*	Total formula weight	56733.9

*Water molecules are not included.