6NZD

Cryo-EM Structure of the Lysosomal Folliculin Complex (FLCN-FNIP2-RagA-RagC-Ragulator)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Ragulator complex protein LAMTOR1	polymer	166	18325.3	1	UniProt (Q6IAA8) Pfam (PF15454) In PDB	Homo sapiens (Human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1,Lipid raft adaptor protein p18,Protein associated with DRMs and endosomes,p27Kip1-releasing factor from RhoA,p27RF-Rho
2	B	Ragulator complex protein LAMTOR2	polymer	127	13645.6	1	UniProt (Q9Y2Q5) Pfam (PF03259) In PDB	Homo sapiens (Human)	Endosomal adaptor protein p14,Late endosomal/lysosomal Mp1-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2,Mitogen-activated protein-binding protein-interacting protein,MAPBP-interacting protein,Roadblock domain-containing protein 3
3	C	Ragulator complex protein LAMTOR3	polymer	124	13637.7	1	UniProt (Q9UHA4) Pfam (PF08923) In PDB	Homo sapiens (Human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3,MEK-binding partner 1,Mp1,Mitogen-activated protein kinase kinase 1-interacting protein 1,Mitogen-activated protein kinase scaffold protein 1
4	D	Ragulator complex protein LAMTOR4	polymer	99	10753.2	1	UniProt (Q0VGL1) In PDB	Homo sapiens (Human)	Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4
5	E	Hepatitis B virus x interacting protein	polymer	173	18178.5	1	UniProt (A0A0C4DGV4) Pfam (PF16672) UniProt (by SIFTS) (O43504) In PDB	Homo sapiens (Human)	Ragulator complex protein LAMTOR5
6	F	Ras-related GTP-binding protein A	polymer	313	36615.2	1	UniProt (Q7L523) Pfam (PF04670) In PDB	Homo sapiens (Human)	RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1
7	G	Ras-related GTP-binding protein C	polymer	404	44758.3	1	UniProt (Q9HB90) Pfam (PF04670) In PDB	Homo sapiens (Human)	RagC,GTPase-interacting protein 2,TIB929
8	H	Folliculin	polymer	622	69143.1	1	UniProt (Q8NFG4) Pfam (PF11704) Pfam (PF16692) In PDB	Homo sapiens (Human)	BHD skin lesion fibrofolliculoma protein,Birt-Hogg-Dube syndrome protein
9	I	Folliculin-interacting protein 2	polymer	1117	122475.4	1	UniProt (Q9P278) Pfam (PF14637) Pfam (PF14638) Pfam (PF14636) In PDB	Homo sapiens (Human)	FNIP1-like protein,O6-methylguanine-induced apoptosis 1 protein
10	F	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	1	Chemie (GDP)
11	G	9-{5-O-[(S)-hydroxy{[(R)-hydroxy(thiophosphonooxy)phosphoryl]oxy}phosphoryl]-alpha-L-arabinofuranosyl}-3,9-dihydro-1H-purine-2,6-dione	non-polymer		540.2	1	Chemie (L8S)

Sequence modifications

A: 5 - 161 (UniProt: Q6IAA8)

PDB	External Database	Details
Ser -4	-	expression tag
Asn -3	-	expression tag
Ala -2	-	expression tag
Glu -1	-	expression tag
Phe 0	-	expression tag
Met 1	-	expression tag
Ala 2	-	expression tag
Cys 3	-	expression tag
Cys 4	-	expression tag

B: 1 - 125 (UniProt: Q9Y2Q5)

PDB	External Database	Details
Gly -1	-	expression tag
Ala 0	-	expression tag

G: 1 - 399 (UniProt: Q9HB90)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Asp -2	-	expression tag
Ser -1	-	expression tag
Arg 0	-	expression tag
Asn 181	Asp 181	engineered mutation

H: 1 - 579 (UniProt: Q8NFG4)

PDB	External Database	Details
Met -42	-	initiating methionine
Ala -41	-	expression tag
Ser -40	-	expression tag
Trp -39	-	expression tag
Ser -38	-	expression tag
His -37	-	expression tag
Pro -36	-	expression tag
Gln -35	-	expression tag
Phe -34	-	expression tag
Glu -33	-	expression tag
Lys -32	-	expression tag
Gly -31	-	expression tag
Gly -30	-	expression tag
Gly -29	-	expression tag
Ala -28	-	expression tag
Arg -27	-	expression tag
Gly -26	-	expression tag
Gly -25	-	expression tag
Ser -24	-	expression tag
Gly -23	-	expression tag
Gly -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Trp -19	-	expression tag
Ser -18	-	expression tag
His -17	-	expression tag
Pro -16	-	expression tag
Gln -15	-	expression tag
Phe -14	-	expression tag
Glu -13	-	expression tag
Lys -12	-	expression tag
Gly -11	-	expression tag
Phe -10	-	expression tag
Asp -9	-	expression tag
Tyr -8	-	expression tag
Lys -7	-	expression tag
Asp -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Lys -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

I: 1 - 1114 (UniProt: Q9P278)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Thr 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	9
	Total formula weight	347532.4
Non-Polymers*	Number of molecules	2
	Total formula weight	983.4
All*	Total formula weight	348515.8

*Water molecules are not included.