6NZD
Cryo-EM Structure of the Lysosomal Folliculin Complex (FLCN-FNIP2-RagA-RagC-Ragulator)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ragulator complex protein LAMTOR1 | polymer | 166 | 18325.3 | 1 | UniProt (Q6IAA8) Pfam (PF15454) | Homo sapiens (Human) | Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1,Lipid raft adaptor protein p18,Protein associated with DRMs and endosomes,p27Kip1-releasing factor from RhoA,p27RF-Rho |
| 2 | B (B) | Ragulator complex protein LAMTOR2 | polymer | 127 | 13645.6 | 1 | UniProt (Q9Y2Q5) Pfam (PF03259) | Homo sapiens (Human) | Endosomal adaptor protein p14,Late endosomal/lysosomal Mp1-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2,Mitogen-activated protein-binding protein-interacting protein,MAPBP-interacting protein,Roadblock domain-containing protein 3 |
| 3 | C (C) | Ragulator complex protein LAMTOR3 | polymer | 124 | 13637.7 | 1 | UniProt (Q9UHA4) Pfam (PF08923) | Homo sapiens (Human) | Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3,MEK-binding partner 1,Mp1,Mitogen-activated protein kinase kinase 1-interacting protein 1,Mitogen-activated protein kinase scaffold protein 1 |
| 4 | D (D) | Ragulator complex protein LAMTOR4 | polymer | 99 | 10753.2 | 1 | UniProt (Q0VGL1) | Homo sapiens (Human) | Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4 |
| 5 | E (E) | Hepatitis B virus x interacting protein | polymer | 173 | 18178.5 | 1 | UniProt (A0A0C4DGV4) Pfam (PF16672) UniProt (by SIFTS) (O43504) | Homo sapiens (Human) | Ragulator complex protein LAMTOR5 |
| 6 | F (F) | Ras-related GTP-binding protein A | polymer | 313 | 36615.2 | 1 | UniProt (Q7L523) Pfam (PF04670) | Homo sapiens (Human) | RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1 |
| 7 | G (G) | Ras-related GTP-binding protein C | polymer | 404 | 44758.3 | 1 | UniProt (Q9HB90) Pfam (PF04670) | Homo sapiens (Human) | RagC,GTPase-interacting protein 2,TIB929 |
| 8 | H (H) | Folliculin | polymer | 622 | 69143.1 | 1 | UniProt (Q8NFG4) Pfam (PF11704) Pfam (PF16692) | Homo sapiens (Human) | BHD skin lesion fibrofolliculoma protein,Birt-Hogg-Dube syndrome protein |
| 9 | I (I) | Folliculin-interacting protein 2 | polymer | 1117 | 122475.4 | 1 | UniProt (Q9P278) Pfam (PF14637) Pfam (PF14638) Pfam (PF14636) | Homo sapiens (Human) | FNIP1-like protein,O6-methylguanine-induced apoptosis 1 protein |
| 10 | J (F) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 11 | K (G) | 9-{5-O-[(S)-hydroxy{[(R)-hydroxy(thiophosphonooxy)phosphoryl]oxy}phosphoryl]-alpha-L-arabinofuranosyl}-3,9-dihydro-1H-purine-2,6-dione | non-polymer | 540.2 | 1 | Chemie (L8S) PubChem (100925674) PubChem (139592514) |
Sequence modifications
A: 5 - 161 (UniProt: Q6IAA8)
B: 1 - 125 (UniProt: Q9Y2Q5)
G: 1 - 399 (UniProt: Q9HB90)
H: 1 - 579 (UniProt: Q8NFG4)
I: 1 - 1114 (UniProt: Q9P278)
| PDB | External Database | Details |
|---|---|---|
| Ser -4 | - | expression tag |
| Asn -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Phe 0 | - | expression tag |
| Met 1 | - | expression tag |
| Ala 2 | - | expression tag |
| Cys 3 | - | expression tag |
| Cys 4 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Arg 0 | - | expression tag |
| Asn 181 | Asp 181 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -42 | - | initiating methionine |
| Ala -41 | - | expression tag |
| Ser -40 | - | expression tag |
| Trp -39 | - | expression tag |
| Ser -38 | - | expression tag |
| His -37 | - | expression tag |
| Pro -36 | - | expression tag |
| Gln -35 | - | expression tag |
| Phe -34 | - | expression tag |
| Glu -33 | - | expression tag |
| Lys -32 | - | expression tag |
| Gly -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Ala -28 | - | expression tag |
| Arg -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Ser -24 | - | expression tag |
| Gly -23 | - | expression tag |
| Gly -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Trp -19 | - | expression tag |
| Ser -18 | - | expression tag |
| His -17 | - | expression tag |
| Pro -16 | - | expression tag |
| Gln -15 | - | expression tag |
| Phe -14 | - | expression tag |
| Glu -13 | - | expression tag |
| Lys -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Tyr -8 | - | expression tag |
| Lys -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 347532.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 983.4 | |
| All* | Total formula weight | 348515.8 |
