6NZD
Cryo-EM Structure of the Lysosomal Folliculin Complex (FLCN-FNIP2-RagA-RagC-Ragulator)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Ragulator complex protein LAMTOR1 | polymer | 166 | 18325.3 | 1 | UniProt (Q6IAA8) Pfam (PF15454) | Homo sapiens (Human) | Late endosomal/lysosomal adaptor and MAPK and MTOR activator 1,Lipid raft adaptor protein p18,Protein associated with DRMs and endosomes,p27Kip1-releasing factor from RhoA,p27RF-Rho |
| 2 | B (B) | Ragulator complex protein LAMTOR2 | polymer | 127 | 13645.6 | 1 | UniProt (Q9Y2Q5) Pfam (PF03259) | Homo sapiens (Human) | Endosomal adaptor protein p14,Late endosomal/lysosomal Mp1-interacting protein,Late endosomal/lysosomal adaptor and MAPK and MTOR activator 2,Mitogen-activated protein-binding protein-interacting protein,MAPBP-interacting protein,Roadblock domain-containing protein 3 |
| 3 | C (C) | Ragulator complex protein LAMTOR3 | polymer | 124 | 13637.7 | 1 | UniProt (Q9UHA4) Pfam (PF08923) | Homo sapiens (Human) | Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3,MEK-binding partner 1,Mp1,Mitogen-activated protein kinase kinase 1-interacting protein 1,Mitogen-activated protein kinase scaffold protein 1 |
| 4 | D (D) | Ragulator complex protein LAMTOR4 | polymer | 99 | 10753.2 | 1 | UniProt (Q0VGL1) | Homo sapiens (Human) | Late endosomal/lysosomal adaptor and MAPK and MTOR activator 4 |
| 5 | E (E) | Hepatitis B virus x interacting protein | polymer | 173 | 18178.5 | 1 | UniProt (A0A0C4DGV4) Pfam (PF16672) UniProt (by SIFTS) (O43504) | Homo sapiens (Human) | Ragulator complex protein LAMTOR5 |
| 6 | F (F) | Ras-related GTP-binding protein A | polymer | 313 | 36615.2 | 1 | UniProt (Q7L523) Pfam (PF04670) | Homo sapiens (Human) | RagA,Adenovirus E3 14.7 kDa-interacting protein 1,FIP-1 |
| 7 | G (G) | Ras-related GTP-binding protein C | polymer | 404 | 44758.3 | 1 | UniProt (Q9HB90) Pfam (PF04670) | Homo sapiens (Human) | RagC,GTPase-interacting protein 2,TIB929 |
| 8 | H (H) | Folliculin | polymer | 622 | 69143.1 | 1 | UniProt (Q8NFG4) Pfam (PF11704) Pfam (PF16692) | Homo sapiens (Human) | BHD skin lesion fibrofolliculoma protein,Birt-Hogg-Dube syndrome protein |
| 9 | I (I) | Folliculin-interacting protein 2 | polymer | 1117 | 122475.4 | 1 | UniProt (Q9P278) Pfam (PF14637) Pfam (PF14638) Pfam (PF14636) | Homo sapiens (Human) | FNIP1-like protein,O6-methylguanine-induced apoptosis 1 protein |
| 10 | J (F) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) | |||
| 11 | K (G) | 9-{5-O-[(S)-hydroxy{[(R)-hydroxy(thiophosphonooxy)phosphoryl]oxy}phosphoryl]-alpha-L-arabinofuranosyl}-3,9-dihydro-1H-purine-2,6-dione | non-polymer | 540.2 | 1 | Chemie (L8S) |
Sequence modifications
A: 5 - 161 (UniProt: Q6IAA8)
B: 1 - 125 (UniProt: Q9Y2Q5)
G: 1 - 399 (UniProt: Q9HB90)
H: 1 - 579 (UniProt: Q8NFG4)
I: 1 - 1114 (UniProt: Q9P278)
| PDB | External Database | Details |
|---|---|---|
| Ser -4 | - | expression tag |
| Asn -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Glu -1 | - | expression tag |
| Phe 0 | - | expression tag |
| Met 1 | - | expression tag |
| Ala 2 | - | expression tag |
| Cys 3 | - | expression tag |
| Cys 4 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -1 | - | expression tag |
| Ala 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Arg 0 | - | expression tag |
| Asn 181 | Asp 181 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Met -42 | - | initiating methionine |
| Ala -41 | - | expression tag |
| Ser -40 | - | expression tag |
| Trp -39 | - | expression tag |
| Ser -38 | - | expression tag |
| His -37 | - | expression tag |
| Pro -36 | - | expression tag |
| Gln -35 | - | expression tag |
| Phe -34 | - | expression tag |
| Glu -33 | - | expression tag |
| Lys -32 | - | expression tag |
| Gly -31 | - | expression tag |
| Gly -30 | - | expression tag |
| Gly -29 | - | expression tag |
| Ala -28 | - | expression tag |
| Arg -27 | - | expression tag |
| Gly -26 | - | expression tag |
| Gly -25 | - | expression tag |
| Ser -24 | - | expression tag |
| Gly -23 | - | expression tag |
| Gly -22 | - | expression tag |
| Gly -21 | - | expression tag |
| Ser -20 | - | expression tag |
| Trp -19 | - | expression tag |
| Ser -18 | - | expression tag |
| His -17 | - | expression tag |
| Pro -16 | - | expression tag |
| Gln -15 | - | expression tag |
| Phe -14 | - | expression tag |
| Glu -13 | - | expression tag |
| Lys -12 | - | expression tag |
| Gly -11 | - | expression tag |
| Phe -10 | - | expression tag |
| Asp -9 | - | expression tag |
| Tyr -8 | - | expression tag |
| Lys -7 | - | expression tag |
| Asp -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Lys -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -2 | - | expression tag |
| Gly -1 | - | expression tag |
| Thr 0 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 9 |
| Total formula weight | 347532.4 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 983.4 | |
| All* | Total formula weight | 348515.8 |
