6NTC

Crystal Structure of G12V HRas-GppNHp bound in complex with the engineered RBD variant 1 of CRAF Kinase protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	GTPase HRas	polymer	171	19388.8	1	UniProt (P01112) Pfam (PF00071) In PDB	Homo sapiens (Human)	H-Ras-1,Ha-Ras,Transforming protein p21,c-H-ras,p21ras
2	B	RAF proto-oncogene serine/threonine-protein kinase	polymer	81	9164.5	1	UniProt (P04049) Pfam (PF02196) In PDB	Homo sapiens (Human)	Proto-oncogene c-RAF,cRaf,Raf-1
3	A	PHOSPHOAMINOPHOSPHONIC ACID-GUANYLATE ESTER	non-polymer		522.2	1	Chemie (GNP)
4	A	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
5	A	MAGNESIUM ION	non-polymer		24.3	1	Chemie (MG)
6		water	water		18.0	5	Chemie (HOH)

Sequence modifications

A: 1 - 166 (UniProt: P01112)

PDB	External Database	Details
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag
Val 12	Gly 12	engineered mutation

B: 55 - 131 (UniProt: P04049)

PDB	External Database	Details
Gly 51	-	expression tag
Ala 52	-	expression tag
Met 53	-	expression tag
Asp 54	-	expression tag
Leu 61	Phe 61	engineered mutation
Gln 65	Lys 65	engineered mutation
Glu 66	Gln 66	engineered mutation
Trp 67	Arg 67	engineered mutation
Lys 71	Asn 71	engineered mutation
Ser 81	Cys 81	engineered mutation
Arg 88	Val 88	engineered mutation
His 89	Arg 89	engineered mutation
Ser 95	Cys 95	engineered mutation
Ser 96	Cys 96	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	28553.3
Non-Polymers*	Number of molecules	3
	Total formula weight	638.6
All*	Total formula weight	29191.9

*Water molecules are not included.