6NTC
Crystal Structure of G12V HRas-GppNHp bound in complex with the engineered RBD variant 1 of CRAF Kinase protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 91.619, 91.619, 151.412 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.344 - 2.900 |
| R-factor | 0.2529 |
| Rwork | 0.252 |
| R-free | 0.27810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g0n |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 151.410 | 3.080 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.140 | |
| Rmeas | 0.144 | |
| Number of reflections | 8883 | 1366 |
| <I/σ(I)> | 15.1 | 0.4 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 18 | 18.7 |
| CC(1/2) | 0.999 | 0.423 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 30% PEG4000, 200mM NH4SO4, 100mM Na CaCo pH 6.5 |
