6NQA
Active state Dot1L bound to the H2B-Ubiquitinated nucleosome, 1-to-1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (I) | 601 DNA Strand 1 | polymer | 146 | 44825.6 | 1 | synthetic construct | ||
| 2 | B (J) | 601 DNA Strand 2 | polymer | 146 | 45305.9 | 1 | synthetic construct | ||
| 3 | C, L (F, B) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 4 | D, K (G, C) | Histone H2A type 1 | polymer | 129 | 13978.2 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 5 | E, J (H, D) | Histone H2B 1.1 | polymer | 122 | 13498.7 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 6 | F (K) | Histone-lysine N-methyltransferase, H3 lysine-79 specific | polymer | 416 | 47432.0 | 1 | UniProt (Q8TEK3) Pfam (PF08123) | Homo sapiens (Human) | DOT1-like protein,Histone H3-K79 methyltransferase,H3-K79-HMTase,Lysine N-methyltransferase 4 |
| 7 | G (L) | Ubiquitin | polymer | 80 | 9036.4 | 1 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (Human) | |
| 8 | H, I (E, A) | Histone H3.2 | polymer | 135 | 15251.8 | 2 | UniProt (P84233) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 9 | M (K) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) PubChem (58633578) PubChem (60038769) PubChem (73897310) PubChem (132988922) PubChem (134692588) PubChem (135030730) PubChem (140459785) PubChem (163046094) PubChem (58766358) PubChem (87224684) PubChem (131697735) PubChem (145712080) PubChem (5136) PubChem (34755) PubChem (9865603) PubChem (12312798) |
Sequence modifications
G, C: 1 - 129 (UniProt: P06897)
H, D: 4 - 125 (UniProt: P02281)
K: 2 - 416 (UniProt: Q8TEK3)
L: 1 - 76 (UniProt: P0CG48)
E, A: 1 - 135 (UniProt: P84233)
| PDB | External Database | Details |
|---|---|---|
| Arg 99 | Gly 100 | engineered mutation |
| Ser 123 | Ala 124 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Thr 32 | Ser 33 | engineered mutation |
| Cys 120 | Lys 121 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Met 0 | - | expression tag |
| Cys 76 | Gly 76 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Nle 79 | Lys 80 | engineered mutation |
| Nle 90 | Met 91 | engineered mutation |
| Ala 102 | Gly 103 | engineered mutation |
| Nle 120 | Met 121 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 254583.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 398.4 | |
| All* | Total formula weight | 254982.3 |
