6NQA
Active state Dot1L bound to the H2B-Ubiquitinated nucleosome, 1-to-1 complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (I) | 601 DNA Strand 1 | polymer | 146 | 44825.6 | 1 | synthetic construct | ||
| 2 | B (J) | 601 DNA Strand 2 | polymer | 146 | 45305.9 | 1 | synthetic construct | ||
| 3 | C, L (F, B) | Histone H4 | polymer | 102 | 11263.2 | 2 | UniProt (P62799) Pfam (PF15511) | Xenopus laevis (African clawed frog) | |
| 4 | D, K (G, C) | Histone H2A type 1 | polymer | 129 | 13978.2 | 2 | UniProt (P06897) Pfam (PF00125) Pfam (PF16211) | Xenopus laevis (African clawed frog) | |
| 5 | E, J (H, D) | Histone H2B 1.1 | polymer | 122 | 13498.7 | 2 | UniProt (P02281) Pfam (PF00125) | Xenopus laevis (African clawed frog) | H2B1.1 |
| 6 | F (K) | Histone-lysine N-methyltransferase, H3 lysine-79 specific | polymer | 416 | 47432.0 | 1 | UniProt (Q8TEK3) Pfam (PF08123) | Homo sapiens (Human) | DOT1-like protein,Histone H3-K79 methyltransferase,H3-K79-HMTase,Lysine N-methyltransferase 4 |
| 7 | G (L) | Ubiquitin | polymer | 80 | 9036.4 | 1 | UniProt (P0CG48) Pfam (PF00240) | Homo sapiens (Human) | |
| 8 | H, I (E, A) | Histone H3.2 | polymer | 135 | 15251.8 | 2 | UniProt (P84233) Pfam (PF00125) | Xenopus laevis (African clawed frog) | |
| 9 | M (K) | S-ADENOSYLMETHIONINE | non-polymer | 398.4 | 1 | Chemie (SAM) |
Sequence modifications
G, C: 1 - 129 (UniProt: P06897)
H, D: 4 - 125 (UniProt: P02281)
K: 2 - 416 (UniProt: Q8TEK3)
L: 1 - 76 (UniProt: P0CG48)
E, A: 1 - 135 (UniProt: P84233)
| PDB | External Database | Details |
|---|---|---|
| Arg 99 | Gly 100 | engineered mutation |
| Ser 123 | Ala 124 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Thr 32 | Ser 33 | engineered mutation |
| Cys 120 | Lys 121 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly -3 | - | expression tag |
| Ser -2 | - | expression tag |
| His -1 | - | expression tag |
| Met 0 | - | expression tag |
| Cys 76 | Gly 76 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Nle 79 | Lys 80 | engineered mutation |
| Nle 90 | Met 91 | engineered mutation |
| Ala 102 | Gly 103 | engineered mutation |
| Nle 120 | Met 121 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 254583.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 398.4 | |
| All* | Total formula weight | 254982.3 |
