6NJ9

Active state Dot1L bound to the H2B-Ubiquitinated nucleosome, 2-to-1 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E	Histone H3.2	polymer	135	15251.8	2	UniProt (P84233) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)
2	B, F	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511) In PDB	Xenopus laevis (African clawed frog)
3	C, G	Histone H2A type 1	polymer	129	13978.2	2	UniProt (P06897) Pfam (PF00125) Pfam (PF16211) In PDB	Xenopus laevis (African clawed frog)
4	D, H	Histone H2B 1.1	polymer	122	13498.7	2	UniProt (P02281) Pfam (PF00125) In PDB	Xenopus laevis (African clawed frog)	H2B1.1
5	I	601 DNA Strand 1	polymer	146	44825.6	1		synthetic construct
6	J	601 DNA Strand 2	polymer	146	45305.9	1		synthetic construct
7	K, M	Histone-lysine N-methyltransferase, H3 lysine-79 specific	polymer	416	47432.0	2	UniProt (Q8TEK3) Pfam (PF08123) In PDB	Homo sapiens (Human)	DOT1-like protein,Histone H3-K79 methyltransferase,H3-K79-HMTase,Lysine N-methyltransferase 4
8	L, N	Ubiquitin	polymer	80	9036.4	2	UniProt (P0CG47) Pfam (PF00240) In PDB	Homo sapiens (Human)
9	K, M	S-ADENOSYLMETHIONINE	non-polymer		398.4	2	Chemie (SAM)

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Nle 79	Lys 80	engineered mutation
Nle 90	Met 91	engineered mutation
Ala 102	Gly 103	engineered mutation
Nle 120	Met 121	engineered mutation

C, G: 1 - 129 (UniProt: P06897)

PDB	External Database	Details
Arg 99	Gly 100	engineered mutation
Ser 123	Ala 124	engineered mutation

D, H: 4 - 125 (UniProt: P02281)

PDB	External Database	Details
Thr 32	Ser 33	engineered mutation
Cys 120	Lys 121	engineered mutation

K, M: 2 - 416 (UniProt: Q8TEK3)

PDB	External Database	Details
Gly 1	-	expression tag

L, N: 1 - 76 (UniProt: P0CG47)

PDB	External Database	Details
Gly -3	-	expression tag
Ser -2	-	expression tag
His -1	-	expression tag
Met 0	-	expression tag
Cys 76	Gly 76	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	14
	Total formula weight	311052.3
Non-Polymers*	Number of molecules	2
	Total formula weight	796.9
All*	Total formula weight	311849.1

*Water molecules are not included.