6MVL

Crystal structure of VISTA bound to a pH-selective antibody Fab fragment

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	V-type immunoglobulin domain-containing suppressor of T-cell activation	polymer	169	19104.3	1	UniProt (Q9H7M9) Pfam (PF07686) In PDB	Homo sapiens (Human)	Platelet receptor Gi24,Stress-induced secreted protein-1,Sisp-1,V-set domain-containing immunoregulatory receptor,V-set immunoregulatory receptor
2	H	Antibody Fab fragment heavy chain	polymer	229	24371.2	1		Homo sapiens (human)
3	L	Antibody Fab fragment light chain	polymer	215	23362.9	1		Homo sapiens (human)
4	A	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
5	A, H, L	SULFATE ION	non-polymer		96.1	14	Chemie (SO4)
6		water	water		18.0	758	Chemie (HOH)

Sequence modifications

A: 32 - 194 (UniProt: Q9H7M9)

PDB	External Database	Details
Gln 91	Asn 91	engineered mutation
Gln 108	Asn 108	engineered mutation
Gln 190	Asn 190	engineered mutation
His 195	-	expression tag
His 196	-	expression tag
His 197	-	expression tag
His 198	-	expression tag
His 199	-	expression tag
His 200	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	66838.3
Non-Polymers*	Number of molecules	15
	Total formula weight	1566.1
All*	Total formula weight	68404.4

*Water molecules are not included.