6MVL
Crystal structure of VISTA bound to a pH-selective antibody Fab fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-08-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.0 |
Spacegroup name | I 21 21 21 |
Unit cell lengths | 66.166, 125.856, 192.040 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 96.020 - 1.610 |
R-factor | 0.184 |
Rwork | 0.182 |
R-free | 0.22500 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 96.020 | 1.807 |
High resolution limit [Å] | 1.606 | 1.606 |
Rmerge | 0.049 | 0.724 |
Number of reflections | 55883 | 2795 |
<I/σ(I)> | 18.8 | 2.2 |
Completeness [%] | 88.6 | 73.9 |
Redundancy | 6.5 | 5.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 295 | 1.8 M ammonium sulfate, 0.1 M phosphate/citrate, pH 4.2 |