6MTI
Synaptotagmin-1 C2A, C2B domains and SNARE-pin proteins (5CCI) individually docked into Cryo-EM map of C2AB-SNARE complexes helically organized on lipid nanotube surface in presence of Mg2+
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | 1, 6 | Synaptotagmin-1 | polymer | 127 | 14654.8 | 2 | UniProt (P21707) Pfam (PF00168) In PDB | Rattus norvegicus (Rat) | Synaptotagmin I,SytI,p65 |
2 | 2, 3, 4, 5 | Synaptotagmin-1 | polymer | 281 | 32247.2 | 4 | UniProt (P21707) Pfam (PF00168) In PDB | Rattus norvegicus (Rat) | Synaptotagmin I,SytI,p65 |
3 | A, E, I, M, Q... | Vesicle-associated membrane protein 2 | polymer | 63 | 7231.1 | 6 | UniProt (P63045) Pfam (PF00957) In PDB | Rattus norvegicus (Rat) | VAMP-2,Synaptobrevin-2 |
4 | B, F, J, N, R... | Syntaxin-1A | polymer | 67 | 7838.0 | 6 | UniProt (P32851) Pfam (PF05739) In PDB | Rattus norvegicus (Rat) | Neuron-specific antigen HPC-1,Synaptotagmin-associated 35 kDa protein,P35A |
5 | C, G, K, O, S... | Synaptosomal-associated protein 25 | polymer | 77 | 9030.1 | 6 | UniProt (P60881) In PDB | Rattus norvegicus (Rat) | SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein |
6 | D, H, L, P, T... | Synaptosomal-associated protein 25 | polymer | 65 | 7471.4 | 6 | UniProt (P60881) In PDB | Rattus norvegicus (Rat) | SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein |
7 | 1, 2, 3, 4, 5... | MAGNESIUM ION | non-polymer | 24.3 | 10 | Chemie (MG) |
Sequence modifications
A, E, I, M, Q, U: 28 - 89 (UniProt: P63045)
B, F, J, N, R, V: 191 - 256 (UniProt: P32851)
D, H, L, P, T, X: 141 - 204 (UniProt: P60881)
PDB | External Database | Details |
---|---|---|
Gly 27 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Met 190 | - | initiating methionine |
PDB | External Database | Details |
---|---|---|
Met 140 | - | initiating methionine |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 30 |
Total formula weight | 347721.3 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 243.1 | |
All* | Total formula weight | 347964.4 |