6MSD

Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome

This is a large structure.

Entity

Chain IDDescriptionTypeChain lengthFormula weightNumber of moleculesDB Name (Accession)Biological sourceDescriptive keywords
U26S proteasome non-ATPase regulatory subunit 1polymer953105958.21
UniProt (Q99460)
Pfam (PF01851)
Pfam (PF13646)
Pfam (PF17781)
Pfam (PF18004)
Homo sapiens (Human)26S proteasome regulatory subunit RPN2,26S proteasome regulatory subunit S1,26S proteasome subunit p112
V26S proteasome non-ATPase regulatory subunit 3polymer53461066.51
UniProt (O43242)
Pfam (PF01399)
Pfam (PF08375)
Homo sapiens (Human)26S proteasome regulatory subunit RPN3,26S proteasome regulatory subunit S3,Proteasome subunit p58
W26S proteasome non-ATPase regulatory subunit 12polymer45652979.41
UniProt (O00232)
Pfam (PF01399)
Pfam (PF18098)
Homo sapiens (Human)26S proteasome regulatory subunit RPN5,26S proteasome regulatory subunit p55
X26S proteasome non-ATPase regulatory subunit 11polymer42247526.71
UniProt (O00231)
Pfam (PF01399)
Pfam (PF18055)
Pfam (PF18503)
Homo sapiens (Human)26S proteasome regulatory subunit RPN6,26S proteasome regulatory subunit S9,26S proteasome regulatory subunit p44.5
Y26S proteasome non-ATPase regulatory subunit 6polymer38945592.31
UniProt (Q15008)
Pfam (PF01399)
Pfam (PF10602)
Homo sapiens (Human)26S proteasome regulatory subunit RPN7,26S proteasome regulatory subunit S10,Breast cancer-associated protein SGA-113M,Phosphonoformate immuno-associated protein 4,Proteasome regulatory particle subunit p44S10,p42A
Z26S proteasome non-ATPase regulatory subunit 7polymer32437086.41
UniProt (P51665)
Pfam (PF01398)
Pfam (PF13012)
Homo sapiens (Human)26S proteasome regulatory subunit RPN8,26S proteasome regulatory subunit S12,Mov34 protein homolog,Proteasome subunit p40
a26S proteasome non-ATPase regulatory subunit 13polymer37642995.41
UniProt (Q9UNM6)
Pfam (PF01399)
Homo sapiens (Human)26S proteasome regulatory subunit RPN9,26S proteasome regulatory subunit S11,26S proteasome regulatory subunit p40.5
b26S proteasome non-ATPase regulatory subunit 4polymer37740781.61
UniProt (P55036)
Pfam (PF02809)
Pfam (PF13519)
Homo sapiens (Human)26S proteasome regulatory subunit RPN10,26S proteasome regulatory subunit S5A,Antisecretory factor 1,ASF,Multiubiquitin chain-binding protein
c26S proteasome non-ATPase regulatory subunit 14polymer30934488.81
UniProt (O00487)
Pfam (PF01398)
Pfam (PF13012)
Homo sapiens (Human)26S proteasome regulatory subunit RPN11,26S proteasome-associated PAD1 homolog 1
d26S proteasome non-ATPase regulatory subunit 8polymer34939536.71
UniProt (P48556)
Pfam (PF10075)
Homo sapiens (Human)26S proteasome regulatory subunit RPN12,26S proteasome regulatory subunit S14,p31
e26S proteasome complex subunit SEM1polymer708284.61
UniProt (P60896)
Pfam (PF05160)
Homo sapiens (Human)26S proteasome complex subunit DSS1,Deleted in split hand/split foot protein 1,Split hand/foot deleted protein 1,Split hand/foot malformation type 1 protein
f26S proteasome non-ATPase regulatory subunit 2polymer908100313.61
UniProt (Q13200)
Pfam (PF01851)
Pfam (PF17781)
Pfam (PF18051)
Homo sapiens (Human)26S proteasome regulatory subunit RPN1,26S proteasome regulatory subunit S2,26S proteasome subunit p97,Protein 55.11,Tumor necrosis factor type 1 receptor-associated protein 2
A26S proteasome regulatory subunit 7polymer43348700.81
UniProt (P35998)
Pfam (PF00004)
Pfam (PF17862)
Homo sapiens (Human)26S proteasome AAA-ATPase subunit RPT1,Proteasome 26S subunit ATPase 2,Protein MSS1
B26S proteasome regulatory subunit 4polymer44049260.51
UniProt (P62191)
Pfam (PF00004)
Pfam (PF16450)
Pfam (PF17862)
Homo sapiens (Human)P26s4,26S proteasome AAA-ATPase subunit RPT2,Proteasome 26S subunit ATPase 1
C26S proteasome regulatory subunit 8polymer39844852.11
UniProt (P62195)
Pfam (PF00004)
Pfam (PF16450)
Pfam (PF17862)
Homo sapiens (Human)26S proteasome AAA-ATPase subunit RPT6,Proteasome 26S subunit ATPase 5,Proteasome subunit p45,Thyroid hormone receptor-interacting protein 1,TRIP1,p45/SUG
D26S proteasome regulatory subunit 6Bpolymer41847426.11
UniProt (P43686)
Pfam (PF00004)
Pfam (PF16450)
Pfam (PF17862)
Homo sapiens (Human)26S proteasome AAA-ATPase subunit RPT3,MB67-interacting protein,MIP224,Proteasome 26S subunit ATPase 4,Tat-binding protein 7,TBP-7
E26S proteasome regulatory subunit 10Bpolymer40345867.01
UniProt (A0A087X2I1)
Pfam (PF00004)
Pfam (PF16450)
Pfam (PF17862)
Homo sapiens (Human)
F26S proteasome regulatory subunit 6Apolymer43949266.51
UniProt (P17980)
Pfam (PF00004)
Pfam (PF16450)
Pfam (PF17862)
Homo sapiens (Human)26S proteasome AAA-ATPase subunit RPT5,Proteasome 26S subunit ATPase 3,Proteasome subunit P50,Tat-binding protein 1,TBP-1
u, wUbiquitinpolymer768604.82
UniProt (P0CG47)
Pfam (PF00240)
Homo sapiens (Human)
G, gProteasome subunit alpha type-6polymer24527301.32
UniProt (P60900)
Pfam (PF00227)
Pfam (PF10584)
Homo sapiens (Human)27 kDa prosomal protein,p27K,Macropain iota chain,Multicatalytic endopeptidase complex iota chain,Proteasome iota chain
H, hProteasome subunit alpha type-2polymer23325796.32
UniProt (P25787)
Pfam (PF00227)
Pfam (PF10584)
Homo sapiens (Human)Macropain subunit C3,Multicatalytic endopeptidase complex subunit C3,Proteasome component C3
I, iProteasome subunit alpha type-4polymer26029394.62
UniProt (P25789)
Pfam (PF00227)
Pfam (PF10584)
Homo sapiens (Human)Macropain subunit C9,Multicatalytic endopeptidase complex subunit C9,Proteasome component C9,Proteasome subunit L
J, jProteasome subunit alpha type-7polymer24727798.72
UniProt (O14818)
Pfam (PF00227)
Pfam (PF10584)
Homo sapiens (Human)Proteasome subunit RC6-1,Proteasome subunit XAPC7
K, kProteasome subunit alpha type-5polymer24026304.82
UniProt (P28066)
Pfam (PF00227)
Pfam (PF10584)
Homo sapiens (Human)Macropain zeta chain,Multicatalytic endopeptidase complex zeta chain,Proteasome zeta chain
L, lProteasome subunit alpha type-1polymer26830150.32
UniProt (P25786)
Pfam (PF00227)
Pfam (PF10584)
Homo sapiens (Human)30 kDa prosomal protein,PROS-30,Macropain subunit C2,Multicatalytic endopeptidase complex subunit C2,Proteasome component C2,Proteasome nu chain
M, mProteasome subunit alpha type-3polymer25428338.12
UniProt (P25788)
Pfam (PF00227)
Pfam (PF10584)
Homo sapiens (Human)Macropain subunit C8,Multicatalytic endopeptidase complex subunit C8,Proteasome component C8
N, nProteasome subunit beta type-6polymer23825246.52
UniProt (P28072)
Pfam (PF00227)
Homo sapiens (Human)Macropain delta chain,Multicatalytic endopeptidase complex delta chain,Proteasome delta chain,Proteasome subunit Y
O, oProteasome subunit beta type-7polymer27629869.22
UniProt (Q99436)
Pfam (PF00227)
Pfam (PF12465)
Homo sapiens (Human)Macropain chain Z,Multicatalytic endopeptidase complex chain Z,Proteasome subunit Z
P, pProteasome subunit beta type-3polymer20422841.72
UniProt (P49720)
Pfam (PF00227)
Homo sapiens (Human)Proteasome chain 13,Proteasome component C10-II,Proteasome theta chain
Q, qProteasome subunit beta type-2polymer20122864.32
UniProt (P49721)
Pfam (PF00227)
Homo sapiens (Human)Macropain subunit C7-I,Multicatalytic endopeptidase complex subunit C7-I,Proteasome component C7-I
R, rProteasome subunit beta type-5polymer26228379.12
UniProt (P28074)
Pfam (PF00227)
Homo sapiens (Human)Macropain epsilon chain,Multicatalytic endopeptidase complex epsilon chain,Proteasome chain 6,Proteasome epsilon chain,Proteasome subunit MB1,Proteasome subunit X
S, sProteasome subunit beta type-1polymer24026391.22
UniProt (P20618)
Pfam (PF00227)
Homo sapiens (Human)Macropain subunit C5,Multicatalytic endopeptidase complex subunit C5,Proteasome component C5,Proteasome gamma chain
T, tProteasome subunit beta type-4polymer26329100.02
UniProt (P28070)
Pfam (PF00227)
Homo sapiens (Human)26 kDa prosomal protein,PROS-26,Macropain beta chain,Multicatalytic endopeptidase complex beta chain,Proteasome beta chain,Proteasome chain 3,HsN3
ZINC IONnon-polymer65.41
ADENOSINE-5'-TRIPHOSPHATEnon-polymer507.24
MAGNESIUM IONnon-polymer24.35
ADENOSINE-5'-DIPHOSPHATEnon-polymer427.22

Sequence viewer

Contents of the asymmetric unit

PolymersNumber of chains48
Total molecular weight1678744.8
Non-Polymers*Number of molecules12
Total molecular weight3070.1
All*Total molecular weight1681814.9
*Water molecules are not included.