6MSD

Cryo-EM structures and dynamics of substrate-engaged human 26S proteasome

This is a large structure.

EM specimen

Vitrificationfalse
Stainingfalse

EM Data Collection

MicroscopeFEI TITAN KRIOS
Electron GunFIELD EMISSION GUN
Accelerating Voltage (kV)300
DetectorGATAN K2 SUMMIT (4k x 4k)

3D Reconstruction

MethodSINGLE PARTICLE
Resolution (Å)3.2
SymmetryC1
CTF correctionPHASE FLIPPING AND AMPLITUDE CORRECTION