6MPX

Twelve chloride ions induce formation and stabilize the NC1 hexamer of collagen IV assembled from transition state trimers

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	fusion protein of non-collagenous domains of collagen type IV	polymer	693	76309.6	1	UniProt (P02462) UniProt (P08572) Pfam (PF01413)	Homo sapiens (Human)	Collagen alpha-2(IV) chain,Collagen alpha-1(IV) chain
2	B, C, D, E, F... (A)	CHLORIDE ION	non-polymer		35.5	6	Chemie (CL)
3	H, I, J, K, L... (A)	SULFATE ION	non-polymer		96.1	8	Chemie (SO4)
4	P (A)	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	1	Chemie (PEG)
5	Q (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	1	Chemie (PGE)
6	R (A)	HEXAETHYLENE GLYCOL	non-polymer		282.3	1	Chemie (P6G)
7	S, T, U, V (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
8	W (A)	water	water		18.0	440	Chemie (HOH)

Sequence modifications

A: -2 - 226 (UniProt: P02462)

PDB	External Database	Details
Ala -16	-	expression tag
Pro -15	-	expression tag
Leu -14	-	expression tag
Ala -13	-	expression tag
Asp -12	-	expression tag
Tyr -11	-	expression tag
Lys -10	-	expression tag
Asp -9	-	expression tag
Asp -8	-	expression tag
Asp -7	-	expression tag
Asp -6	-	expression tag
Lys -5	-	expression tag
Leu -4	-	expression tag
Ala -3	-	expression tag

A: 229 - 449 (UniProt: P08572)

PDB	External Database	Details
Ala 450	-	linker
Pro 451	-	linker

A: 452 - 676 (UniProt: P02462)

PDB	External Database	Details
Gly 227	-	linker
Thr 228	-	linker

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	76309.6
Non-Polymers*	Number of molecules	21
	Total formula weight	1888.2
All*	Total formula weight	78197.8

*Water molecules are not included.