6MN4
Crystal structure of aminoglycoside acetyltransferase AAC(3)-IVa, H154A mutant, in complex with apramycin
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Aminoglycoside N(3)-acetyltransferase, AAC(3)-IVa | polymer | 260 | 28057.1 | 6 | UniProt (Q306W4) Pfam (PF02522) | Escherichia coli | |
| 2 | CA, G, HA, O, T... (E, A, F, B, C...) | APRAMYCIN | non-polymer | 539.6 | 6 | Chemie (AM2) | |||
| 3 | H, P, U, Y (A, B, C, D) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 4 | DA, I, J, K, L... (E, A, B) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 6 | Chemie (EDO) | |||
| 5 | EA, IA, M, R, V... (E, F, A, B, C...) | PHOSPHATE ION | non-polymer | 95.0 | 6 | Chemie (PO4) | |||
| 6 | BA, GA, JA, N, S... (D, E, F, A, B...) | 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID | non-polymer | 238.3 | 6 | Chemie (EPE) | |||
| 7 | AA, FA (D, E) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 8 | KA, LA, MA, NA, OA... (A, B, C, D, E...) | water | water | 18.0 | 339 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 1 - 258 (UniProt: Q306W4)
| PDB | External Database | Details |
|---|---|---|
| Gln -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ala 154 | His 154 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 168342.4 | |
| Non-Polymers* | Number of molecules | 30 |
| Total formula weight | 6055.4 | |
| All* | Total formula weight | 174397.8 |
