6MN4

Crystal structure of aminoglycoside acetyltransferase AAC(3)-IVa, H154A mutant, in complex with apramycin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0008080	molecular_function	N-acetyltransferase activity
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0046677	biological_process	response to antibiotic
A	0046872	molecular_function	metal ion binding
B	0008080	molecular_function	N-acetyltransferase activity
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0046677	biological_process	response to antibiotic
B	0046872	molecular_function	metal ion binding
C	0008080	molecular_function	N-acetyltransferase activity
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0046677	biological_process	response to antibiotic
C	0046872	molecular_function	metal ion binding
D	0008080	molecular_function	N-acetyltransferase activity
D	0016740	molecular_function	transferase activity
D	0016746	molecular_function	acyltransferase activity
D	0046677	biological_process	response to antibiotic
D	0046872	molecular_function	metal ion binding
E	0008080	molecular_function	N-acetyltransferase activity
E	0016740	molecular_function	transferase activity
E	0016746	molecular_function	acyltransferase activity
E	0046677	biological_process	response to antibiotic
E	0046872	molecular_function	metal ion binding
F	0008080	molecular_function	N-acetyltransferase activity
F	0016740	molecular_function	transferase activity
F	0016746	molecular_function	acyltransferase activity
F	0046677	biological_process	response to antibiotic
F	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	13
Details	binding site for residue AM2 A 301

Chain	Residue
A	TRP63
A	HOH406
A	HOH421
A	HOH430
A	HOH432
A	ASP67
A	HIS124
A	ARG168
A	HIS169
A	GLU185
A	ASP187
A	HIS188
A	GLU249

---

site_id	AC2
Number of Residues	4
Details	binding site for residue ZN A 302

Chain	Residue
A	CYS189
A	HIS241
A	CYS247
A	CYS250

---

site_id	AC3
Number of Residues	3
Details	binding site for residue EDO A 303

Chain	Residue
A	LYS7
A	GLU46
A	ALA50

---

site_id	AC4
Number of Residues	2
Details	binding site for residue EDO A 304

Chain	Residue
A	ASP39
A	GLU46

---

site_id	AC5
Number of Residues	3
Details	binding site for residue EDO A 305

Chain	Residue
A	PRO73
A	LYS96
A	ARG97

---

site_id	AC6
Number of Residues	1
Details	binding site for residue EDO A 306

Chain	Residue
A	LEU121

---

site_id	AC7
Number of Residues	5
Details	binding site for residue PO4 A 307

Chain	Residue
A	SER30
A	PHE31
A	ARG32
A	GLY83
A	VAL84

---

site_id	AC8
Number of Residues	7
Details	binding site for residue EPE A 308

Chain	Residue
A	PHE31
A	ARG35
A	PRO36
A	LEU37
A	GLU38
A	GLY40
B	ALA74

---

site_id	AC9
Number of Residues	10
Details	binding site for residue AM2 B 301

Chain	Residue
B	TRP63
B	ASP67
B	HIS124
B	PRO167
B	ARG168
B	HIS169
B	GLU185
B	ASP187
B	GLU249
B	HOH412

---

site_id	AD1
Number of Residues	4
Details	binding site for residue ZN B 302

Chain	Residue
B	CYS189
B	HIS241
B	CYS247
B	CYS250

---

site_id	AD2
Number of Residues	4
Details	binding site for residue EDO B 303

Chain	Residue
B	ARG192
B	ASP235
B	LEU237
B	LEU240

---

site_id	AD3
Number of Residues	6
Details	binding site for residue PO4 B 304

Chain	Residue
B	SER30
B	PHE31
B	ARG32
B	GLY83
B	VAL84
B	HOH403

---

site_id	AD4
Number of Residues	8
Details	binding site for residue EPE B 305

Chain	Residue
A	ALA74
B	GLU4
B	PHE31
B	ARG35
B	PRO36
B	LEU37
B	GLU38
B	GLY40

---

site_id	AD5
Number of Residues	9
Details	binding site for residue AM2 C 301

Chain	Residue
C	TRP63
C	ASP67
C	HIS124
C	ARG168
C	HIS169
C	GLU185
C	ASP187
C	HIS188
C	HOH412

---

site_id	AD6
Number of Residues	4
Details	binding site for residue ZN C 302

Chain	Residue
C	CYS189
C	HIS241
C	CYS247
C	CYS250

---

site_id	AD7
Number of Residues	5
Details	binding site for residue PO4 C 303

Chain	Residue
C	SER30
C	PHE31
C	ARG32
C	GLY83
C	VAL84

---

site_id	AD8
Number of Residues	7
Details	binding site for residue EPE C 304

Chain	Residue
C	GLU4
C	PHE31
C	ARG35
C	PRO36
C	LEU37
C	GLU38
C	GLY40

---

site_id	AD9
Number of Residues	10
Details	binding site for residue AM2 D 301

Chain	Residue
D	HIS169
D	GLU185
D	ASP187
D	GLU249
D	HOH404
D	HOH411
D	TRP63
D	ASP67
D	HIS124
D	ARG168

---

site_id	AE1
Number of Residues	4
Details	binding site for residue ZN D 302

Chain	Residue
D	CYS189
D	HIS241
D	CYS247
D	CYS250

---

site_id	AE2
Number of Residues	6
Details	binding site for residue PO4 D 303

Chain	Residue
D	SER30
D	PHE31
D	ARG32
D	GLY83
D	VAL84
D	HOH403

---

site_id	AE3
Number of Residues	3
Details	binding site for residue GOL D 304

Chain	Residue
C	PRO236
D	ARG198
D	ARG234

---

site_id	AE4
Number of Residues	7
Details	binding site for residue EPE D 305

Chain	Residue
D	GLU4
D	PHE31
D	ARG35
D	PRO36
D	LEU37
D	GLU38
D	GLY40

---

site_id	AE5
Number of Residues	8
Details	binding site for residue AM2 E 301

Chain	Residue
E	TRP63
E	HIS124
E	THR151
E	ARG168
E	HIS169
E	GLU185
E	ASP187
E	HIS188

---

site_id	AE6
Number of Residues	2
Details	binding site for residue EDO E 302

Chain	Residue
E	LYS96
E	ARG97

---

site_id	AE7
Number of Residues	5
Details	binding site for residue PO4 E 303

Chain	Residue
E	SER30
E	PHE31
E	ARG32
E	GLY83
E	VAL84

---

site_id	AE8
Number of Residues	1
Details	binding site for residue GOL E 304

Chain	Residue
E	THR75

---

site_id	AE9
Number of Residues	8
Details	binding site for residue EPE E 305

Chain	Residue
D	ALA74
E	GLU4
E	PHE31
E	ARG35
E	PRO36
E	LEU37
E	GLU38
E	GLY40

---

site_id	AF1
Number of Residues	8
Details	binding site for residue AM2 F 301

Chain	Residue
F	TRP63
F	HIS124
F	ARG168
F	HIS169
F	GLU185
F	ASP187
F	HIS188
F	GLU249

---

site_id	AF2
Number of Residues	7
Details	binding site for residue PO4 F 302

Chain	Residue
F	PHE31
F	ARG32
F	GLY83
F	VAL84
F	HOH401
F	HOH408
F	HOH413

---

site_id	AF3
Number of Residues	4
Details	binding site for residue EPE F 303

Chain	Residue
F	PHE31
F	ARG35
F	PRO36
F	LEU37

---