6MDP

The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Vesicle-fusing ATPase	polymer	768	85509.2	6	UniProt (P18708) Pfam (PF02359) Pfam (PF02933) Pfam (PF00004) Pfam (PF17862) Pfam (PF21964)	Cricetulus griseus (Chinese hamster)	N-ethylmaleimide-sensitive fusion protein,NEM-sensitive fusion protein,Vesicular-fusion protein NSF
2	G (H)	Synaptosomal-associated protein 25	polymer	207	23512.4	1	UniProt (P60881) Pfam (PF00835)	Rattus norvegicus (Rat)	SNAP-25,Super protein,SUP,Synaptosomal-associated 25 kDa protein
3	H, Q (A, E)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
4	I, J, K, L, M... (A, B, C, D, E...)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	9	Chemie (ATP)

Sequence modifications

A, B, C, D, E, F: 1 - 723 (UniProt: P18708)

PDB	External Database	Details
Met -23	-	initiating methionine
Gly -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
Asp -15	-	expression tag
Tyr -14	-	expression tag
Asp -13	-	expression tag
Ile -12	-	expression tag
Pro -11	-	expression tag
Thr -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Gly -2	-	expression tag
Ala -1	-	expression tag
His 0	-	expression tag
Ile 458	Lys 458	conflict
Tyr 724	-	expression tag
Arg 725	-	expression tag
Val 726	-	expression tag
Arg 727	-	expression tag
Lys 728	-	expression tag
Phe 729	-	expression tag
Leu 730	-	expression tag
Ala 731	-	expression tag
Leu 732	-	expression tag
Leu 733	-	expression tag
Arg 734	-	expression tag
Glu 735	-	expression tag
Glu 736	-	expression tag
Gly 737	-	expression tag
Ala 738	-	expression tag
Ser 739	-	expression tag
Pro 740	-	expression tag
Leu 741	-	expression tag
Asp 742	-	expression tag
Phe 743	-	expression tag
Asp 744	-	expression tag

H: 1 - 204 (UniProt: P60881)

PDB	External Database	Details
Met -2	-	initiating methionine
Ala -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	536567.7
Non-Polymers*	Number of molecules	11
	Total formula weight	5419.0
All*	Total formula weight	541986.8

*Water molecules are not included.