Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

6MDP

The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000149	molecular_function	SNARE binding
A	0001921	biological_process	positive regulation of receptor recycling
A	0005524	molecular_function	ATP binding
A	0005737	cellular_component	cytoplasm
A	0005794	cellular_component	Golgi apparatus
A	0005795	cellular_component	Golgi stack
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0006813	biological_process	potassium ion transport
A	0006886	biological_process	intracellular protein transport
A	0006891	biological_process	intra-Golgi vesicle-mediated transport
A	0015031	biological_process	protein transport
A	0016787	molecular_function	hydrolase activity
A	0016887	molecular_function	ATP hydrolysis activity
A	0017075	molecular_function	syntaxin-1 binding
A	0019901	molecular_function	protein kinase binding
A	0019905	molecular_function	syntaxin binding
A	0030165	molecular_function	PDZ domain binding
A	0030496	cellular_component	midbody
A	0035255	molecular_function	ionotropic glutamate receptor binding
A	0035494	biological_process	SNARE complex disassembly
A	0042802	molecular_function	identical protein binding
A	0043001	biological_process	Golgi to plasma membrane protein transport
A	0044877	molecular_function	protein-containing complex binding
A	0045732	biological_process	positive regulation of protein catabolic process
A	0046872	molecular_function	metal ion binding
A	0140545	molecular_function	ATP-dependent protein disaggregase activity
B	0000149	molecular_function	SNARE binding
B	0001921	biological_process	positive regulation of receptor recycling
B	0005524	molecular_function	ATP binding
B	0005737	cellular_component	cytoplasm
B	0005794	cellular_component	Golgi apparatus
B	0005795	cellular_component	Golgi stack
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0006813	biological_process	potassium ion transport
B	0006886	biological_process	intracellular protein transport
B	0006891	biological_process	intra-Golgi vesicle-mediated transport
B	0015031	biological_process	protein transport
B	0016787	molecular_function	hydrolase activity
B	0016887	molecular_function	ATP hydrolysis activity
B	0017075	molecular_function	syntaxin-1 binding
B	0019901	molecular_function	protein kinase binding
B	0019905	molecular_function	syntaxin binding
B	0030165	molecular_function	PDZ domain binding
B	0030496	cellular_component	midbody
B	0035255	molecular_function	ionotropic glutamate receptor binding
B	0035494	biological_process	SNARE complex disassembly
B	0042802	molecular_function	identical protein binding
B	0043001	biological_process	Golgi to plasma membrane protein transport
B	0044877	molecular_function	protein-containing complex binding
B	0045732	biological_process	positive regulation of protein catabolic process
B	0046872	molecular_function	metal ion binding
B	0140545	molecular_function	ATP-dependent protein disaggregase activity
C	0000149	molecular_function	SNARE binding
C	0001921	biological_process	positive regulation of receptor recycling
C	0005524	molecular_function	ATP binding
C	0005737	cellular_component	cytoplasm
C	0005794	cellular_component	Golgi apparatus
C	0005795	cellular_component	Golgi stack
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0006813	biological_process	potassium ion transport
C	0006886	biological_process	intracellular protein transport
C	0006891	biological_process	intra-Golgi vesicle-mediated transport
C	0015031	biological_process	protein transport
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0017075	molecular_function	syntaxin-1 binding
C	0019901	molecular_function	protein kinase binding
C	0019905	molecular_function	syntaxin binding
C	0030165	molecular_function	PDZ domain binding
C	0030496	cellular_component	midbody
C	0035255	molecular_function	ionotropic glutamate receptor binding
C	0035494	biological_process	SNARE complex disassembly
C	0042802	molecular_function	identical protein binding
C	0043001	biological_process	Golgi to plasma membrane protein transport
C	0044877	molecular_function	protein-containing complex binding
C	0045732	biological_process	positive regulation of protein catabolic process
C	0046872	molecular_function	metal ion binding
C	0140545	molecular_function	ATP-dependent protein disaggregase activity
D	0000149	molecular_function	SNARE binding
D	0001921	biological_process	positive regulation of receptor recycling
D	0005524	molecular_function	ATP binding
D	0005737	cellular_component	cytoplasm
D	0005794	cellular_component	Golgi apparatus
D	0005795	cellular_component	Golgi stack
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0006813	biological_process	potassium ion transport
D	0006886	biological_process	intracellular protein transport
D	0006891	biological_process	intra-Golgi vesicle-mediated transport
D	0015031	biological_process	protein transport
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0017075	molecular_function	syntaxin-1 binding
D	0019901	molecular_function	protein kinase binding
D	0019905	molecular_function	syntaxin binding
D	0030165	molecular_function	PDZ domain binding
D	0030496	cellular_component	midbody
D	0035255	molecular_function	ionotropic glutamate receptor binding
D	0035494	biological_process	SNARE complex disassembly
D	0042802	molecular_function	identical protein binding
D	0043001	biological_process	Golgi to plasma membrane protein transport
D	0044877	molecular_function	protein-containing complex binding
D	0045732	biological_process	positive regulation of protein catabolic process
D	0046872	molecular_function	metal ion binding
D	0140545	molecular_function	ATP-dependent protein disaggregase activity
E	0000149	molecular_function	SNARE binding
E	0001921	biological_process	positive regulation of receptor recycling
E	0005524	molecular_function	ATP binding
E	0005737	cellular_component	cytoplasm
E	0005794	cellular_component	Golgi apparatus
E	0005795	cellular_component	Golgi stack
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0006813	biological_process	potassium ion transport
E	0006886	biological_process	intracellular protein transport
E	0006891	biological_process	intra-Golgi vesicle-mediated transport
E	0015031	biological_process	protein transport
E	0016787	molecular_function	hydrolase activity
E	0016887	molecular_function	ATP hydrolysis activity
E	0017075	molecular_function	syntaxin-1 binding
E	0019901	molecular_function	protein kinase binding
E	0019905	molecular_function	syntaxin binding
E	0030165	molecular_function	PDZ domain binding
E	0030496	cellular_component	midbody
E	0035255	molecular_function	ionotropic glutamate receptor binding
E	0035494	biological_process	SNARE complex disassembly
E	0042802	molecular_function	identical protein binding
E	0043001	biological_process	Golgi to plasma membrane protein transport
E	0044877	molecular_function	protein-containing complex binding
E	0045732	biological_process	positive regulation of protein catabolic process
E	0046872	molecular_function	metal ion binding
E	0140545	molecular_function	ATP-dependent protein disaggregase activity
F	0000149	molecular_function	SNARE binding
F	0001921	biological_process	positive regulation of receptor recycling
F	0005524	molecular_function	ATP binding
F	0005737	cellular_component	cytoplasm
F	0005794	cellular_component	Golgi apparatus
F	0005795	cellular_component	Golgi stack
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0006813	biological_process	potassium ion transport
F	0006886	biological_process	intracellular protein transport
F	0006891	biological_process	intra-Golgi vesicle-mediated transport
F	0015031	biological_process	protein transport
F	0016787	molecular_function	hydrolase activity
F	0016887	molecular_function	ATP hydrolysis activity
F	0017075	molecular_function	syntaxin-1 binding
F	0019901	molecular_function	protein kinase binding
F	0019905	molecular_function	syntaxin binding
F	0030165	molecular_function	PDZ domain binding
F	0030496	cellular_component	midbody
F	0035255	molecular_function	ionotropic glutamate receptor binding
F	0035494	biological_process	SNARE complex disassembly
F	0042802	molecular_function	identical protein binding
F	0043001	biological_process	Golgi to plasma membrane protein transport
F	0044877	molecular_function	protein-containing complex binding
F	0045732	biological_process	positive regulation of protein catabolic process
F	0046872	molecular_function	metal ion binding
F	0140545	molecular_function	ATP-dependent protein disaggregase activity
H	0000149	molecular_function	SNARE binding
H	0001917	cellular_component	photoreceptor inner segment
H	0005249	molecular_function	voltage-gated potassium channel activity
H	0005484	molecular_function	SNAP receptor activity
H	0005515	molecular_function	protein binding
H	0005737	cellular_component	cytoplasm
H	0005768	cellular_component	endosome
H	0005802	cellular_component	trans-Golgi network
H	0005856	cellular_component	cytoskeleton
H	0005886	cellular_component	plasma membrane
H	0005938	cellular_component	cell cortex
H	0006887	biological_process	exocytosis
H	0007269	biological_process	neurotransmitter secretion
H	0007409	biological_process	axonogenesis
H	0007616	biological_process	long-term memory
H	0007626	biological_process	locomotory behavior
H	0008021	cellular_component	synaptic vesicle
H	0008076	cellular_component	voltage-gated potassium channel complex
H	0008289	molecular_function	lipid binding
H	0008306	biological_process	associative learning
H	0010975	biological_process	regulation of neuron projection development
H	0015629	cellular_component	actin cytoskeleton
H	0016020	cellular_component	membrane
H	0016079	biological_process	synaptic vesicle exocytosis
H	0016082	biological_process	synaptic vesicle priming
H	0016197	biological_process	endosomal transport
H	0017022	molecular_function	myosin binding
H	0017075	molecular_function	syntaxin-1 binding
H	0019904	molecular_function	protein domain specific binding
H	0030027	cellular_component	lamellipodium
H	0030175	cellular_component	filopodium
H	0030182	biological_process	neuron differentiation
H	0030424	cellular_component	axon
H	0030426	cellular_component	growth cone
H	0031083	cellular_component	BLOC-1 complex
H	0031201	cellular_component	SNARE complex
H	0031629	biological_process	synaptic vesicle fusion to presynaptic active zone membrane
H	0031982	cellular_component	vesicle
H	0032024	biological_process	positive regulation of insulin secretion
H	0035493	biological_process	SNARE complex assembly
H	0036477	cellular_component	somatodendritic compartment
H	0042734	cellular_component	presynaptic membrane
H	0043005	cellular_component	neuron projection
H	0043025	cellular_component	neuronal cell body
H	0043195	cellular_component	terminal bouton
H	0044295	cellular_component	axonal growth cone
H	0044325	molecular_function	transmembrane transporter binding
H	0045202	cellular_component	synapse
H	0046887	biological_process	positive regulation of hormone secretion
H	0048306	molecular_function	calcium-dependent protein binding
H	0048471	cellular_component	perinuclear region of cytoplasm
H	0048787	cellular_component	presynaptic active zone membrane
H	0048791	biological_process	calcium ion-regulated exocytosis of neurotransmitter
H	0051963	biological_process	regulation of synapse assembly
H	0060291	biological_process	long-term synaptic potentiation
H	0070032	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex
H	0070033	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex
H	0070044	cellular_component	synaptobrevin 2-SNAP-25-syntaxin-1a complex
H	0070201	biological_process	regulation of establishment of protein localization
H	0071805	biological_process	potassium ion transmembrane transport
H	0097470	cellular_component	ribbon synapse
H	0098793	cellular_component	presynapse
H	0098794	cellular_component	postsynapse
H	0098888	cellular_component	extrinsic component of presynaptic membrane
H	0098967	biological_process	exocytic insertion of neurotransmitter receptor to postsynaptic membrane
H	0098978	cellular_component	glutamatergic synapse
H	0099525	biological_process	presynaptic dense core vesicle exocytosis
H	0099590	biological_process	neurotransmitter receptor internalization

Functional Information from PDB Data

site_id	AC1
Number of Residues	9
Details	binding site for residue ADP A 801

Chain	Residue
A	GLY221
A	PRO262
A	CYS264
A	GLY265
A	LYS266
A	THR267
A	LEU268
A	ILE406
A	ALA439

site_id	AC2
Number of Residues	13
Details	binding site for residue ATP A 802

Chain	Residue
A	MET504
A	ASN505
A	GLY506
A	ILE508
A	PRO545
A	GLY548
A	LYS549
A	THR550
A	ALA551
A	LEU552
A	SER647
A	ILE707
A	LYS708

site_id	AC3
Number of Residues	13
Details	binding site for residue ATP B 801

Chain	Residue
B	TYR502
B	ASN505
B	GLY506
B	ILE508
B	TRP510
B	HIS546
B	SER547
B	GLY548
B	LYS549
B	THR550
B	ALA551
B	ASP604
B	ILE707

site_id	AC4
Number of Residues	16
Details	binding site for residue ATP B 802

Chain	Residue
A	ARG385
A	ARG388
B	GLY221
B	PRO262
B	GLY263
B	CYS264
B	GLY265
B	LYS266
B	THR267
B	LEU268
B	GLU329
B	ASN374
B	ILE406
B	GLY438
B	ALA439
B	GLU442

site_id	AC5
Number of Residues	15
Details	binding site for residue ATP C 801

Chain	Residue
B	LYS631
C	TYR502
C	ASN505
C	GLY506
C	ILE507
C	ILE508
C	PRO545
C	HIS546
C	SER547
C	GLY548
C	LYS549
C	THR550
C	ASP604
C	ILE707
C	LYS708

site_id	AC6
Number of Residues	17
Details	binding site for residue ATP C 802

Chain	Residue
B	LEU355
B	ASP359
B	ARG385
B	ARG388
C	GLY221
C	PRO262
C	GLY263
C	CYS264
C	GLY265
C	LYS266
C	THR267
C	LEU268
C	GLU329
C	ASN374
C	ILE406
C	GLY438
C	ALA439

site_id	AC7
Number of Residues	13
Details	binding site for residue ATP D 801

Chain	Residue
C	LYS631
D	MET504
D	ASN505
D	GLY506
D	ILE508
D	PRO545
D	HIS546
D	GLY548
D	LYS549
D	THR550
D	ALA551
D	ILE707
D	LYS708

site_id	AC8
Number of Residues	19
Details	binding site for residue ATP D 802

Chain	Residue
C	ARG388
D	ILE220
D	GLY221
D	PRO262
D	GLY263
D	CYS264
D	GLY265
D	LYS266
D	THR267
D	LEU268
D	GLU329
D	MET372
D	ASN374
D	ILE406
D	GLY438
D	GLU442
C	LEU355
C	ASP359
C	ARG385

site_id	AC9
Number of Residues	17
Details	binding site for residue ATP E 801

Chain	Residue
D	LYS631
E	MET504
E	ASN505
E	GLY506
E	ILE507
E	ILE508
E	TRP510
E	PRO545
E	HIS546
E	GLY548
E	LYS549
E	THR550
E	ALA551
E	ASP604
E	ILE707
E	LYS708
E	LEU711

site_id	AD1
Number of Residues	13
Details	binding site for residue ADP E 802

Chain	Residue
D	ARG388
E	ILE220
E	PRO262
E	GLY263
E	CYS264
E	GLY265
E	LYS266
E	THR267
E	LEU268
E	ASN374
E	ILE406
E	HIS410
E	ALA439

site_id	AD2
Number of Residues	14
Details	binding site for residue ATP F 801

Chain	Residue
E	LYS631
F	MET504
F	ASN505
F	GLY506
F	ILE508
F	PRO545
F	GLY548
F	LYS549
F	THR550
F	ALA551
F	LEU552
F	ASP604
F	SER647
F	ILE707

Functional Information from PROSITE/UniProt

site_id	PS00674
Number of Residues	19
Details	AAA AAA-protein family signature. IlVIgMTNrpdlIDeALl.R

Chain	Residue	Details
A	ILE367-ARG385

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	SITE: (Microbial infection) Cleavage; by C.botulinum neurotoxin type E (BoNT/E) => ECO:0000269\|PubMed:8243676, ECO:0000269\|PubMed:8294407, ECO:0000305\|PubMed:8103915

Chain	Residue	Details
H	ARG180
E	PRO545
F	ASN505
F	PRO545
A	PRO545
B	ASN505
B	PRO545
C	ASN505
C	PRO545
D	ASN505
D	PRO545
E	ASN505

site_id	SWS_FT_FI2
Number of Residues	1
Details	SITE: (Microbial infection) Cleavage; by C.botulinum neurotoxin type A (BoNT/A, botA) => ECO:0000269\|PubMed:8243676, ECO:0000269\|PubMed:8294407, ECO:0000305\|PubMed:8103915

Chain	Residue	Details
H	GLN197
B	THR550
C	THR550
D	THR550
E	THR550
F	THR550

site_id	SWS_FT_FI3
Number of Residues	1
Details	MOD_RES: Phosphothreonine; by PKC and PKA => ECO:0000269\|PubMed:12459461

Chain	Residue	Details
H	THR138
B	LYS105
C	LYS105
D	LYS105
E	LYS105
F	LYS105

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000250\|UniProtKB:P60879

Chain	Residue	Details
H	SER154
B	SER207
C	SER207
D	SER207
E	SER207
F	SER207

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PKC => ECO:0000269\|PubMed:12459461

Chain	Residue	Details
H	SER187
B	TYR259
C	TYR259
D	TYR259
E	TYR259
F	TYR259

site_id	SWS_FT_FI6
Number of Residues	4
Details	LIPID: S-palmitoyl cysteine => ECO:0000250\|UniProtKB:P60879

Chain	Residue	Details
H	CYS85
H	PHE88
H	CYS90
H	CYS92
E	SER569
F	SER569

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
A	LYS549	electrostatic stabiliser
A	THR550	electrostatic stabiliser
A	ASP603	electrostatic stabiliser
A	ASP604	proton acceptor
A	LYS631	electrostatic stabiliser
A	LYS708	electrostatic stabiliser

site_id	MCSA2
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
B	LYS549	electrostatic stabiliser
B	THR550	electrostatic stabiliser
B	ASP603	electrostatic stabiliser
B	ASP604	proton acceptor
B	LYS631	electrostatic stabiliser
B	LYS708	electrostatic stabiliser

site_id	MCSA3
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
C	LYS549	electrostatic stabiliser
C	THR550	electrostatic stabiliser
C	ASP603	electrostatic stabiliser
C	ASP604	proton acceptor
C	LYS631	electrostatic stabiliser
C	LYS708	electrostatic stabiliser

site_id	MCSA4
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
D	LYS549	electrostatic stabiliser
D	THR550	electrostatic stabiliser
D	ASP603	electrostatic stabiliser
D	ASP604	proton acceptor
D	LYS631	electrostatic stabiliser
D	LYS708	electrostatic stabiliser

site_id	MCSA5
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
E	LYS549	electrostatic stabiliser
E	THR550	electrostatic stabiliser
E	ASP603	electrostatic stabiliser
E	ASP604	proton acceptor
E	LYS631	electrostatic stabiliser
E	LYS708	electrostatic stabiliser

site_id	MCSA6
Number of Residues	6
Details	M-CSA 642

Chain	Residue	Details
F	LYS549	electrostatic stabiliser
F	THR550	electrostatic stabiliser
F	ASP603	electrostatic stabiliser
F	ASP604	proton acceptor
F	LYS631	electrostatic stabiliser
F	LYS708	electrostatic stabiliser

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)