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- EMDB-9103: The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus... -

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Entry
Database: EMDB / ID: EMD-9103
TitleThe D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)
Map dataThe unsharpened map.
Sample
  • Complex: 20S supercomplex consisting of soluble neuronal SNARE complex, alpha-SNAP, and N-ethylmaleimide sensitive factor (NSF)
    • Complex: N-ethylmaleimide sensitive factor
      • Protein or peptide: Vesicle-fusing ATPase
    • Complex: Synaptosomal-associated protein 25
      • Protein or peptide: Synaptosomal-associated protein 25
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
KeywordsSNARE / NSF / SNAP / ATPase / AAA / disassembly / synapse / membrane fusion / exocytosis / HYDROLASE
Function / homology
Function and homology information


BLOC-1 complex / SNARE complex disassembly / exocytic insertion of neurotransmitter receptor to postsynaptic membrane / Other interleukin signaling / synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex / synaptobrevin 2-SNAP-25-syntaxin-1a complex / synaptic vesicle fusion to presynaptic active zone membrane / synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex / calcium ion-regulated exocytosis of neurotransmitter / Glutamate Neurotransmitter Release Cycle ...BLOC-1 complex / SNARE complex disassembly / exocytic insertion of neurotransmitter receptor to postsynaptic membrane / Other interleukin signaling / synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex / synaptobrevin 2-SNAP-25-syntaxin-1a complex / synaptic vesicle fusion to presynaptic active zone membrane / synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex / calcium ion-regulated exocytosis of neurotransmitter / Glutamate Neurotransmitter Release Cycle / Norepinephrine Neurotransmitter Release Cycle / Acetylcholine Neurotransmitter Release Cycle / Serotonin Neurotransmitter Release Cycle / GABA synthesis, release, reuptake and degradation / presynaptic dense core vesicle exocytosis / Dopamine Neurotransmitter Release Cycle / extrinsic component of presynaptic membrane / ribbon synapse / regulation of establishment of protein localization / SNAP receptor activity / SNARE complex / neurotransmitter receptor internalization / ATP-dependent protein disaggregase activity / intra-Golgi vesicle-mediated transport / Golgi to plasma membrane protein transport / Golgi stack / positive regulation of hormone secretion / neurotransmitter secretion / vesicle-fusing ATPase / syntaxin-1 binding / Neutrophil degranulation / SNARE complex assembly / endosomal transport / synaptic vesicle priming / myosin binding / regulation of synapse assembly / positive regulation of receptor recycling / regulation of neuron projection development / exocytosis / associative learning / synaptic vesicle exocytosis / voltage-gated potassium channel activity / long-term memory / axonal growth cone / ionotropic glutamate receptor binding / voltage-gated potassium channel complex / somatodendritic compartment / photoreceptor inner segment / axonogenesis / SNARE binding / filopodium / PDZ domain binding / locomotory behavior / intracellular protein transport / long-term synaptic potentiation / trans-Golgi network / potassium ion transport / positive regulation of insulin secretion / terminal bouton / neuron differentiation / calcium-dependent protein binding / positive regulation of protein catabolic process / actin cytoskeleton / synaptic vesicle / lamellipodium / presynapse / presynaptic membrane / growth cone / cell cortex / midbody / chromatin extrusion motor activity / ATP-dependent H2AZ histone chaperone activity / ATP-dependent H3-H4 histone complex chaperone activity / cohesin loader activity / DNA clamp loader activity / vesicle / transmembrane transporter binding / cytoskeleton / postsynapse / endosome / neuron projection / protein domain specific binding / axon / neuronal cell body / lipid binding / synapse / protein-containing complex binding / protein kinase binding / perinuclear region of cytoplasm / glutamatergic synapse / ATP hydrolysis activity / ATP binding / identical protein binding / membrane / metal ion binding / plasma membrane / cytosol / cytoplasm
Similarity search - Function
: / NSF, AAA+ ATPase lid domain / Vesicle-fusing ATPase / Synaptosomal-associated protein 25 / SNAP-25 domain / SNAP-25 family / Helical region found in SNAREs / t-SNARE coiled-coil homology domain profile. / Target SNARE coiled-coil homology domain / Cell division protein 48 (CDC48), N-terminal domain ...: / NSF, AAA+ ATPase lid domain / Vesicle-fusing ATPase / Synaptosomal-associated protein 25 / SNAP-25 domain / SNAP-25 family / Helical region found in SNAREs / t-SNARE coiled-coil homology domain profile. / Target SNARE coiled-coil homology domain / Cell division protein 48 (CDC48), N-terminal domain / CDC48, N-terminal subdomain / Cell division protein 48 (CDC48) N-terminal domain / CDC48, domain 2 / Cell division protein 48 (CDC48), domain 2 / Cell division protein 48 (CDC48) domain 2 / CDC48 domain 2-like superfamily / Aspartate decarboxylase-like domain superfamily / AAA ATPase, AAA+ lid domain / AAA+ lid domain / ATPase, AAA-type, conserved site / AAA-protein family signature. / ATPase family associated with various cellular activities (AAA) / ATPase, AAA-type, core / ATPases associated with a variety of cellular activities / AAA+ ATPase domain / P-loop containing nucleoside triphosphate hydrolase
Similarity search - Domain/homology
Vesicle-fusing ATPase / Synaptosomal-associated protein 25
Similarity search - Component
Biological speciesRattus norvegicus (Norway rat) / Cricetulus griseus (Chinese hamster)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.8 Å
AuthorsWhite KI / Zhao M
Funding support United States, 1 items
OrganizationGrant numberCountry
National Institutes of Health/National Human Genome Research Institute (NIH/NHGRI)R37MH63105 United States
CitationJournal: Elife / Year: 2018
Title: Structural principles of SNARE complex recognition by the AAA+ protein NSF.
Authors: K Ian White / Minglei Zhao / Ucheor B Choi / Richard A Pfuetzner / Axel T Brunger /
Abstract: The recycling of SNARE proteins following complex formation and membrane fusion is an essential process in eukaryotic trafficking. A highly conserved AAA+ protein, NSF (-ethylmaleimide sensitive ...The recycling of SNARE proteins following complex formation and membrane fusion is an essential process in eukaryotic trafficking. A highly conserved AAA+ protein, NSF (-ethylmaleimide sensitive factor) and an adaptor protein, SNAP (soluble NSF attachment protein), disassemble the SNARE complex. We report electron-cryomicroscopy structures of the complex of NSF, αSNAP, and the full-length soluble neuronal SNARE complex (composed of syntaxin-1A, synaptobrevin-2, SNAP-25A) in the presence of ATP under non-hydrolyzing conditions at ~3.9 Å resolution. These structures reveal electrostatic interactions by which two αSNAP molecules interface with a specific surface of the SNARE complex. This interaction positions the SNAREs such that the 15 N-terminal residues of SNAP-25A are loaded into the D1 ring pore of NSF via a spiral pattern of interactions between a conserved tyrosine NSF residue and SNAP-25A backbone atoms. This loading process likely precedes ATP hydrolysis. Subsequent ATP hydrolysis then drives complete disassembly.
History
DepositionSep 4, 2018-
Header (metadata) releaseSep 19, 2018-
Map releaseSep 19, 2018-
UpdateMar 13, 2024-
Current statusMar 13, 2024Processing site: RCSB / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0135
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.0135
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: PDB-6mdp
  • Surface level: 0.0135
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_9103.png

Downloads & links

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Map

FileDownload / File: emd_9103.map.gz / Format: CCP4 / Size: 46.4 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThe unsharpened map.
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.31 Å/pix.
x 230 pix.
= 301.3 Å		1.31 Å/pix.
x 230 pix.
= 301.3 Å		1.31 Å/pix.
x 230 pix.
= 301.3 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.31 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.0135 / Movie #1: 0.0135
Minimum - Maximum-0.009235976 - 0.043628827
Average (Standard dev.)0.00005939628 (±0.0024336001)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions230230230
Spacing230230230
CellA=B=C: 301.3 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.311.311.31
M x/y/z230230230
origin x/y/z0.0000.0000.000
length x/y/z301.300301.300301.300
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS230230230
D min/max/mean-0.0090.0440.000

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Supplemental data

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Additional map: The sharpened map.

Fileemd_9103_additional.map
AnnotationThe sharpened map.
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 20S supercomplex consisting of soluble neuronal SNARE complex, al...

EntireName: 20S supercomplex consisting of soluble neuronal SNARE complex, alpha-SNAP, and N-ethylmaleimide sensitive factor (NSF)
Components
  • Complex: 20S supercomplex consisting of soluble neuronal SNARE complex, alpha-SNAP, and N-ethylmaleimide sensitive factor (NSF)
    • Complex: N-ethylmaleimide sensitive factor
      • Protein or peptide: Vesicle-fusing ATPase
    • Complex: Synaptosomal-associated protein 25
      • Protein or peptide: Synaptosomal-associated protein 25
  • Ligand: ADENOSINE-5'-DIPHOSPHATE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE

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Supramolecule #1: 20S supercomplex consisting of soluble neuronal SNARE complex, al...

SupramoleculeName: 20S supercomplex consisting of soluble neuronal SNARE complex, alpha-SNAP, and N-ethylmaleimide sensitive factor (NSF)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#2
Molecular weightTheoretical: 23.625 KDa

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Supramolecule #2: N-ethylmaleimide sensitive factor

SupramoleculeName: N-ethylmaleimide sensitive factor / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Rattus norvegicus (Norway rat)

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Supramolecule #3: Synaptosomal-associated protein 25

SupramoleculeName: Synaptosomal-associated protein 25 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #2
Source (natural)Organism: Rattus norvegicus (Norway rat)

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Macromolecule #1: Vesicle-fusing ATPase

MacromoleculeName: Vesicle-fusing ATPase / type: protein_or_peptide / ID: 1 / Number of copies: 6 / Enantiomer: LEVO / EC number: vesicle-fusing ATPase
Source (natural)Organism: Cricetulus griseus (Chinese hamster)
Molecular weightTheoretical: 85.509227 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MGHHHHHHDY DIPTTENLYF QGAHMAGRSM QAARCPTDEL SLSNCAVVSE KDYQSGQHVI VRTSPNHKYI FTLRTHPSVV PGSVAFSLP QRKWAGLSIG QEIEVALYSF DKAKQCIGTM TIEIDFLQKK NIDSNPYDTD KMAAEFIQQF NNQAFSVGQQ L VFSFNDKL ...String:
MGHHHHHHDY DIPTTENLYF QGAHMAGRSM QAARCPTDEL SLSNCAVVSE KDYQSGQHVI VRTSPNHKYI FTLRTHPSVV PGSVAFSLP QRKWAGLSIG QEIEVALYSF DKAKQCIGTM TIEIDFLQKK NIDSNPYDTD KMAAEFIQQF NNQAFSVGQQ L VFSFNDKL FGLLVKDIEA MDPSILKGEP ASGKRQKIEV GLVVGNSQVA FEKAENSSLN LIGKAKTKEN RQSIINPDWN FE KMGIGGL DKEFSDIFRR AFASRVFPPE IVEQMGCKHV KGILLYGPPG CGKTLLARQI GKMLNAREPK VVNGPEILNK YVG ESEANI RKLFADAEEE QRRLGANSGL HIIIFDEIDA ICKQRGSMAG STGVHDTVVN QLLSKIDGVE QLNNILVIGM TNRP DLIDE ALLRPGRLEV KMEIGLPDEK GRLQILHIHT ARMRGHQLLS ADVDIKELAV ETKNFSGAEL EGLVRAAQST AMNRH IIAS TKVEVDMEKA ESLQVTRGDF LASLENDIKP AFGTNQEDYA SYIMNGIIKW GDPVTRVLDD GELLVQQTKN SDRTPL VSV LLEGPPHSGK TALAAKIAEE SNFPFIKICS PDKMIGFSET AKCQAMKKIF DDAYKSQLSC VVVDDIERLL DYVPIGP RF SNLVLQALLV LLKKAPPQGR KLLIIGTTSR KDVLQEMEML NAFSTTIHVP NIATGEQLLE ALELLGNFKD KERTTIAQ Q VKGKKVWIGI KKLLMLIEMS LQMDPEYRVR KFLALLREEG ASPLDFD

UniProtKB: Vesicle-fusing ATPase

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Macromolecule #2: Synaptosomal-associated protein 25

MacromoleculeName: Synaptosomal-associated protein 25 / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Rattus norvegicus (Norway rat)
Molecular weightTheoretical: 23.512387 KDa
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MASMAEDADM RNELEEMQRR ADQLADESLE STRRMLQLVE ESKDAGIRTL VMLDEQGEQL DRVEEGMNHI NQDMKEAEKN LKDLGKCCG LFICPCNKLK SSDAYKKAWG NNQDGVVASQ PARVVDEREQ MAISGGFIRR VTNDARENEM DENLEQVSGI I GNLRHMAL ...String:
MASMAEDADM RNELEEMQRR ADQLADESLE STRRMLQLVE ESKDAGIRTL VMLDEQGEQL DRVEEGMNHI NQDMKEAEKN LKDLGKCCG LFICPCNKLK SSDAYKKAWG NNQDGVVASQ PARVVDEREQ MAISGGFIRR VTNDARENEM DENLEQVSGI I GNLRHMAL DMGNEIDTQN RQIDRIMEKA DSNKTRIDEA NQRATKMLG

UniProtKB: Synaptosomal-associated protein 25

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Macromolecule #3: ADENOSINE-5'-DIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-DIPHOSPHATE / type: ligand / ID: 3 / Number of copies: 2 / Formula: ADP
Molecular weightTheoretical: 427.201 Da
Chemical component information

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

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Macromolecule #4: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 4 / Number of copies: 9 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration15 mg/mL
BufferpH: 8
Component:
ConcentrationFormulaName
0.05 MC4H11NO3Tris HCl
0.15 MNaClSodium chloride
0.001 MC10H16N5O13P3Adenosine triphosphate
0.001 MC10H16N2O8Ethylenediaminetetraacetic acid
0.001 MC4H10O2S2Dithiothreitol
0.05 % v/vNonidet P-40
GridModel: Quantifoil R1.2/1.3 / Material: COPPER / Mesh: 400 / Support film - Material: CARBON / Support film - topology: HOLEY ARRAY
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 293 K / Instrument: FEI VITROBOT MARK I / Details: Blot for 3.5 seconds before plunging..

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: SUPER-RESOLUTION / Digitization - Frames/image: 2-40 / Number grids imaged: 2 / Number real images: 5418 / Average exposure time: 10.0 sec. / Average electron dose: 58.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 70.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 3.0 µm / Nominal defocus min: 1.5 µm
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 475680
Startup modelType of model: PDB ENTRY
PDB model - PDB ID:

3j96

Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.8 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1) / Number images used: 184555
Initial angle assignmentType: PROJECTION MATCHING / Software - Name: RELION (ver. 2.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 2.1)
Final 3D classificationNumber classes: 4 / Software - Name: RELION (ver. 2.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelPDB ID:

3j96


Chain - Source name: PDB / Chain - Initial model type: experimental model
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-6mdp:
The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2)

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