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- EMDB-9100: The 20S supercomplex engaging the SNAP-25 N-terminus (class 1) -

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Entry
Database: EMDB / ID: 9100
TitleThe 20S supercomplex engaging the SNAP-25 N-terminus (class 1)
Map dataThe unsharpened map.
Sample20S supercomplex consisting of soluble neuronal SNARE complex, alpha-SNAP, and N-ethylmaleimide sensitive factor (NSF)
  • N-ethylmaleimide sensitive factor
  • (Synaptosomal-associated protein ...) x 2
  • (Syntaxin-1A) x 2
  • (Vesicle-associated membrane protein ...) x 2
  • (Alpha-soluble NSF attachment ...) x 2
  • Vesicle-fusing ATPase
  • (ligand) x 2
Function / homologySyntaxin / CDC48 domain 2-like superfamily / Target SNARE coiled-coil homology domain / NSF attachment protein / Cell division protein 48 (CDC48), domain 2 / Cell division protein 48 (CDC48), N-terminal domain / Synaptobrevin / SNAP-25 family / SNAP-25 / ATPase family associated with various cellular activities (AAA) ...Syntaxin / CDC48 domain 2-like superfamily / Target SNARE coiled-coil homology domain / NSF attachment protein / Cell division protein 48 (CDC48), domain 2 / Cell division protein 48 (CDC48), N-terminal domain / Synaptobrevin / SNAP-25 family / SNAP-25 / ATPase family associated with various cellular activities (AAA) / Vesicle-fusing ATPase / Synaptosomal-associated protein 25 / Synaptobrevin / Vesicle-associated membrane protein 2 / CDC48, N-terminal subdomain / Syntaxin 1A / P-loop containing nucleoside triphosphate hydrolase / Synaptobrevin/Vesicle-associated membrane protein / Tetratricopeptide-like helical domain superfamily / SNARE / Aspartate decarboxylase-like domain superfamily / Syntaxin/epimorphin, conserved site / Syntaxin, N-terminal domain / CDC48, domain 2 / ATPase, AAA-type, conserved site / ATPase, AAA-type, core / SNARE domain / Synaptobrevin signature. / AAA-protein family signature. / Syntaxin / epimorphin family signature. / GABA synthesis, release, reuptake and degradation / Clathrin-mediated endocytosis / Cargo recognition for clathrin-mediated endocytosis / Retrograde transport at the Trans-Golgi-Network / Intra-Golgi traffic / COPI-dependent Golgi-to-ER retrograde traffic / COPI-mediated anterograde transport / LGI-ADAM interactions / Other interleukin signaling / Golgi Associated Vesicle Biogenesis / Lysosome Vesicle Biogenesis / Clathrin derived vesicle budding / Acetylcholine Neurotransmitter Release Cycle / Dopamine Neurotransmitter Release Cycle / Glutamate Neurotransmitter Release Cycle / COPII-mediated vesicle transport / Norepinephrine Neurotransmitter Release Cycle / Serotonin Neurotransmitter Release Cycle / v-SNARE coiled-coil homology domain profile. / t-SNARE coiled-coil homology domain profile. / AAA+ ATPase domain / soluble NSF attachment protein activity / SNARE complex disassembly / regulation of delayed rectifier potassium channel activity / exocytic insertion of neurotransmitter receptor to postsynaptic membrane / storage vacuole / myosin head/neck binding / regulation of synaptic vesicle priming / anchored component of presynaptic membrane / zymogen granule membrane / growth hormone secretion / synaptobrevin 2-SNAP-25-syntaxin-1a complex / synaptobrevin 2-SNAP-25-syntaxin-1a-complexin II complex / neurotransmitter transport / positive regulation of norepinephrine secretion / synaptobrevin 2-SNAP-25-syntaxin-1a-complexin I complex / presynaptic active zone membrane / synaptic vesicle fusion to presynaptic active zone membrane / positive regulation of catecholamine secretion / synaptic vesicle docking / SNARE complex assembly / regulation of synaptic vesicle recycling / synaptic vesicle priming / intracellular organelle / positive regulation of neurotransmitter secretion / apical protein localization / protein-containing complex disassembly / Golgi to plasma membrane protein transport / positive regulation of calcium ion-dependent exocytosis / calcium ion-regulated exocytosis of neurotransmitter / regulation of synapse assembly / vesicle docking / vesicle-fusing ATPase / protein localization to membrane / positive regulation of hormone secretion / chloride channel inhibitor activity / vesicle fusion / integral component of synaptic vesicle membrane / response to gravity / sleep / SNAP receptor activity / positive regulation of ATPase activity / regulation of neuron projection development / regulation of exocytosis / synaptic vesicle exocytosis / positive regulation of receptor recycling / SNARE complex / positive regulation of intracellular protein transport / membrane fusion / syntaxin-1 binding
Function and homology information
SourceCricetulus griseus (Chinese hamster) / Rattus norvegicus (Norway rat)
Methodsingle particle reconstruction / cryo EM / 4.4 Å resolution
AuthorsWhite KI / Zhao M / Brunger AT
CitationJournal: Elife / Year: 2018
Title: Structural principles of SNARE complex recognition by the AAA+ protein NSF.
Authors: K Ian White / Minglei Zhao / Ucheor B Choi / Richard A Pfuetzner / Axel T Brunger
Abstract: The recycling of SNARE proteins following complex formation and membrane fusion is an essential process in eukaryotic trafficking. A highly conserved AAA+ protein, NSF (-ethylmaleimide sensitive ...The recycling of SNARE proteins following complex formation and membrane fusion is an essential process in eukaryotic trafficking. A highly conserved AAA+ protein, NSF (-ethylmaleimide sensitive factor) and an adaptor protein, SNAP (soluble NSF attachment protein), disassemble the SNARE complex. We report electron-cryomicroscopy structures of the complex of NSF, αSNAP, and the full-length soluble neuronal SNARE complex (composed of syntaxin-1A, synaptobrevin-2, SNAP-25A) in the presence of ATP under non-hydrolyzing conditions at ~3.9 Å resolution. These structures reveal electrostatic interactions by which two αSNAP molecules interface with a specific surface of the SNARE complex. This interaction positions the SNAREs such that the 15 N-terminal residues of SNAP-25A are loaded into the D1 ring pore of NSF via a spiral pattern of interactions between a conserved tyrosine NSF residue and SNAP-25A backbone atoms. This loading process likely precedes ATP hydrolysis. Subsequent ATP hydrolysis then drives complete disassembly.
Validation ReportPDB-ID: 6mdm

SummaryFull reportAbout validation report
DateDeposition: Sep 4, 2018 / Header (metadata) release: Sep 19, 2018 / Map release: Sep 19, 2018 / Last update: Sep 26, 2018

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.0135
  • Imaged by UCSF Chimera
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  • Surface view colored by cylindrical radius
  • Surface level: 0.0135
  • Imaged by UCSF Chimera
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  • Surface view with fitted model
  • Atomic models: : PDB-6mdm
  • Surface level: 0.0135
  • Imaged by UCSF Chimera
  • Download
Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

Downloads & links

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Map

Fileemd_9100.map.gz (map file in CCP4 format, 48669 KB)
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
230 pix
1.31 Å/pix.
= 301.3 Å
230 pix
1.31 Å/pix.
= 301.3 Å
230 pix
1.31 Å/pix.
= 301.3 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.31 Å
Density
Contour Level:0.0135 (by author), 0.0135 (movie #1):
Minimum - Maximum-0.008086054 - 0.040476423
Average (Standard dev.)0.00031600829 (0.002695571)
Details

EMDB XML:

Space Group Number1
Map Geometry
Axis orderXYZ
Dimensions230230230
Origin0.00.00.0
Limit229.0229.0229.0
Spacing230230230
CellA=B=C: 301.3 Å
α=β=γ: 90.0 deg.

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.311.311.31
M x/y/z230230230
origin x/y/z0.0000.0000.000
length x/y/z301.300301.300301.300
α/β/γ90.00090.00090.000
start NX/NY/NZ
NX/NY/NZ
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS230230230
D min/max/mean-0.0080.0400.000

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Supplemental data

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Sample components

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Entire 20S supercomplex consisting of soluble neuronal SNARE complex, al...

EntireName: 20S supercomplex consisting of soluble neuronal SNARE complex, alpha-SNAP, and N-ethylmaleimide sensitive factor (NSF)
Number of components: 13

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Component #1: protein, 20S supercomplex consisting of soluble neuronal SNARE co...

ProteinName: 20S supercomplex consisting of soluble neuronal SNARE complex, alpha-SNAP, and N-ethylmaleimide sensitive factor (NSF)
Recombinant expression: No
MassTheoretical: 33.25 kDa

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Component #2: protein, N-ethylmaleimide sensitive factor

ProteinName: N-ethylmaleimide sensitive factor / Recombinant expression: No
SourceSpecies: Cricetulus griseus (Chinese hamster)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #3: protein, Synaptosomal-associated protein 25

ProteinName: Synaptosomal-associated protein 25SNAP25 / Recombinant expression: No
SourceSpecies: Rattus norvegicus (Norway rat)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #4: protein, Syntaxin-1A

ProteinName: Syntaxin-1A / Recombinant expression: No
SourceSpecies: Rattus norvegicus (Norway rat)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #5: protein, Vesicle-associated membrane protein 2

ProteinName: Vesicle-associated membrane protein 2Vesicle-associated membrane protein
Recombinant expression: No
SourceSpecies: Rattus norvegicus (Norway rat)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #6: protein, Alpha-soluble NSF attachment protein

ProteinName: Alpha-soluble NSF attachment protein / Recombinant expression: No
SourceSpecies: Rattus norvegicus (Norway rat)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #7: protein, Vesicle-fusing ATPase

ProteinName: Vesicle-fusing ATPase / Number of Copies: 6 / Recombinant expression: No
MassTheoretical: 85.509227 kDa
SourceSpecies: Cricetulus griseus (Chinese hamster)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #8: protein, Synaptosomal-associated protein 25

ProteinName: Synaptosomal-associated protein 25SNAP25 / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 23.512387 kDa
SourceSpecies: Rattus norvegicus (Norway rat)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #9: protein, Syntaxin-1A

ProteinName: Syntaxin-1A / Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 29.634129 kDa
SourceSpecies: Rattus norvegicus (Norway rat)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #10: protein, Vesicle-associated membrane protein 2

ProteinName: Vesicle-associated membrane protein 2Vesicle-associated membrane protein
Number of Copies: 1 / Recombinant expression: No
MassTheoretical: 12.981304 kDa
SourceSpecies: Rattus norvegicus (Norway rat)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #11: protein, Alpha-soluble NSF attachment protein

ProteinName: Alpha-soluble NSF attachment protein / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 35.598223 kDa
SourceSpecies: Rattus norvegicus (Norway rat)
Source (engineered)Expression System: Escherichia coli (E. coli)

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Component #12: ligand, ADENOSINE-5'-TRIPHOSPHATE

LigandName: ADENOSINE-5'-TRIPHOSPHATE / Number of Copies: 9 / Recombinant expression: No
MassTheoretical: 0.507181 kDa

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Component #13: ligand, ADENOSINE-5'-DIPHOSPHATE

LigandName: ADENOSINE-5'-DIPHOSPHATE / Number of Copies: 2 / Recombinant expression: No
MassTheoretical: 0.427201 kDa

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Experimental details

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Sample preparation

SpecimenSpecimen state: particle / Method: cryo EM
Sample solutionSpecimen conc.: 15 mg/ml / pH: 8
Support filmNot available
VitrificationInstrument: FEI VITROBOT MARK I / Cryogen name: ETHANE / Temperature: 293 K / Humidity: 100 % / Details: Blot for 3.5 seconds before plunging..

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
ImagingMicroscope: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Electron dose: 58 e/Å2 / Illumination mode: FLOOD BEAM
LensCs: 2.7 mm / Imaging mode: BRIGHT FIELD / Defocus: 1500.0 - 3000.0 nm
Specimen HolderModel: FEI TITAN KRIOS AUTOGRID HOLDER
CameraDetector: GATAN K2 SUMMIT (4k x 4k)

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Image acquisition

Image acquisitionNumber of digital images: 5418

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Image processing

ProcessingMethod: single particle reconstruction / Applied symmetry: C1 (asymmetric) / Number of projections: 55150
3D reconstructionSoftware: RELION
CTF correction: CTF correction was carried out in Relion with reconstruction step.
Resolution: 4.4 Å / Resolution method: FSC 0.143 CUT-OFF
FSC plot
(resolution estimation)

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Atomic model buiding

Modeling #1Refinement protocol: rigid body / Refinement space: REAL
Input PDB model: 3J96
Output model

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