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- EMDB-0127: Human nuclear RNA exosome EXO-14 complex -

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Basic information

Entry
Database: EMDB / ID: EMD-0127
TitleHuman nuclear RNA exosome EXO-14 complex
Map dataCryo-EM reconstruction of the human nuclear RNA exosome EXO-14 complex
Sample
  • Complex: Human nuclear RNA exosome EXO-14 complex
    • Complex: Human nuclear RNA exosome EXO-14 complex
      • DNA: x 1 types
    • Complex: Human nuclear RNA exosome EXO-14 complex
      • Protein or peptide: x 14 types
Biological speciessynthetic construct (others) / Homo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 6.25 Å
AuthorsGerlach P / Schuller JM / Falk S / Basquin J / Conti E
CitationJournal: Elife / Year: 2018
Title: Distinct and evolutionary conserved structural features of the human nuclear exosome complex.
Authors: Piotr Gerlach / Jan M Schuller / Fabien Bonneau / Jérôme Basquin / Peter Reichelt / Sebastian Falk / Elena Conti /
Abstract: The nuclear RNA exosome complex mediates the processing of structured RNAs and the decay of aberrant non-coding RNAs, an important function particularly in human cells. Most mechanistic studies to ...The nuclear RNA exosome complex mediates the processing of structured RNAs and the decay of aberrant non-coding RNAs, an important function particularly in human cells. Most mechanistic studies to date have focused on the yeast system. Here, we reconstituted and studied the properties of a recombinant 14-subunit human nuclear exosome complex. In biochemical assays, the human exosome embeds a longer RNA channel than its yeast counterpart. The 3.8 Å resolution cryo-EM structure of the core complex bound to a single-stranded RNA reveals that the RNA channel path is formed by two distinct features of the hDIS3 exoribonuclease: an open conformation and a domain organization more similar to bacterial RNase II than to yeast Rrp44. The cryo-EM structure of the holo-complex shows how obligate nuclear cofactors position the hMTR4 helicase at the entrance of the core complex, suggesting a striking structural conservation from lower to higher eukaryotes.
History
DepositionJul 12, 2018-
Header (metadata) releaseJul 25, 2018-
Map releaseAug 1, 2018-
UpdateAug 8, 2018-
Current statusAug 8, 2018Processing site: PDBe / Status: Released

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Structure visualization

Movie
  • Surface view with section colored by density value
  • Surface level: 0.015
  • Imaged by UCSF Chimera
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  • Surface view colored by radius
  • Surface level: 0.015
  • Imaged by UCSF Chimera
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Movie viewer
Structure viewerEM map:
SurfViewMolmilJmol/JSmol
Supplemental images

emd_0127.png

Downloads & links

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Map

FileDownload / File: emd_0127.map.gz / Format: CCP4 / Size: 59.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationCryo-EM reconstruction of the human nuclear RNA exosome EXO-14 complex
Voxel sizeX=Y=Z: 1.35 Å
Density
Contour LevelBy AUTHOR: 0.015 / Movie #1: 0.015
Minimum - Maximum-0.066417865 - 0.118277155
Average (Standard dev.)0.00018988713 (±0.0038044313)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions250250250
Spacing250250250
CellA=B=C: 337.5 Å
α=β=γ: 90.0 °

CCP4 map header:

modeImage stored as Reals
Å/pix. X/Y/Z1.351.351.35
M x/y/z250250250
origin x/y/z0.0000.0000.000
length x/y/z337.500337.500337.500
α/β/γ90.00090.00090.000
MAP C/R/S123
start NC/NR/NS000
NC/NR/NS250250250
D min/max/mean-0.0660.1180.000

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Supplemental data

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Sample components

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Entire : Human nuclear RNA exosome EXO-14 complex

EntireName: Human nuclear RNA exosome EXO-14 complex
Components
  • Complex: Human nuclear RNA exosome EXO-14 complex
    • Complex: Human nuclear RNA exosome EXO-14 complex
      • DNA: U44 ssRNA
    • Complex: Human nuclear RNA exosome EXO-14 complex
      • Protein or peptide: EXOSC9
      • Protein or peptide: EXOSC4
      • Protein or peptide: EXOSC8
      • Protein or peptide: EXOSC5
      • Protein or peptide: EXOSC7
      • Protein or peptide: EXOSC6
      • Protein or peptide: EXOSC3
      • Protein or peptide: EXOSC2
      • Protein or peptide: EXOSC1
      • Protein or peptide: DIS3
      • Protein or peptide: MPP6
      • Protein or peptide: EXOSC10
      • Protein or peptide: C1D
      • Protein or peptide: MTR4

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Supramolecule #1: Human nuclear RNA exosome EXO-14 complex

SupramoleculeName: Human nuclear RNA exosome EXO-14 complex / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all

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Supramolecule #2: Human nuclear RNA exosome EXO-14 complex

SupramoleculeName: Human nuclear RNA exosome EXO-14 complex / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #15
Source (natural)Organism: synthetic construct (others)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Supramolecule #3: Human nuclear RNA exosome EXO-14 complex

SupramoleculeName: Human nuclear RNA exosome EXO-14 complex / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1-#14
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)

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Macromolecule #1: EXOSC9

MacromoleculeName: EXOSC9 / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MKETPLSNCE RRFLLRAIEE KKRLDGRQTY DYRNIRISFG TDYGCCIVEL GKTRVLGQVS CELVSPKLN RATEGILFFN LELSQMAAPA FEPGRQSDLL VKLNRLMERC LRNSKCIDTE S LCVVAGEK VWQIRVDLHL LNHDGNIIDA ASIAAIVALC HFRRPDVSVQ ...String:
MKETPLSNCE RRFLLRAIEE KKRLDGRQTY DYRNIRISFG TDYGCCIVEL GKTRVLGQVS CELVSPKLN RATEGILFFN LELSQMAAPA FEPGRQSDLL VKLNRLMERC LRNSKCIDTE S LCVVAGEK VWQIRVDLHL LNHDGNIIDA ASIAAIVALC HFRRPDVSVQ GDEVTLYTPE ER DPVPLSI HHMPICVSFA FFQQGTYLLV DPNEREERVM DGLLVIAMNK HREICTIQSS GGI MLLKDQ VLRCSKIAGV KVAEITELIL KALENDQKVR KEGGKFGFAE SIANQRITAF KMEK APIDT SDVEEKAEEI IAEAEPPSEV VSTPVLWTPG TAQIGEGVEN SWGDLEDSEK EDDEG GGDQ AIILDGIKMD TGVEVSDIGS QDAPIILSDS EEEEMIILEP DKNPKKIRTQ TTSAKQ EKA PSKKPVKRRK KKRAAN

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Macromolecule #2: EXOSC4

MacromoleculeName: EXOSC4 / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAGLELLSDQ GYRVDGRRAG ELRKIQARMG VFAQADGSAY IEQGNTKALA VVYGPHEIRG SRARALPDR ALVNCQYSSA TFSTGERKRR PHGDRKSCEM GLQLRQTFEA AILTQLHPRS Q IDIYVQVL QADGGTYAAC VNAATLAVLD AGIPMRDFVC ACSAGFVDGT ...String:
MAGLELLSDQ GYRVDGRRAG ELRKIQARMG VFAQADGSAY IEQGNTKALA VVYGPHEIRG SRARALPDR ALVNCQYSSA TFSTGERKRR PHGDRKSCEM GLQLRQTFEA AILTQLHPRS Q IDIYVQVL QADGGTYAAC VNAATLAVLD AGIPMRDFVC ACSAGFVDGT ALADLSHVEE AA GGPQLAL ALLPASGQIA LLEMDARLHE DHLERVLEAA AQAARDVHTL LDRVVRQHVR EAS ILLGD

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Macromolecule #3: EXOSC8

MacromoleculeName: EXOSC8 / type: protein_or_peptide / ID: 3 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAAGFKTVEP LEYYRRFLKE NCRPDGRELG EFRTTTVNIG SISTADGSAL VKLGNTTVIC GVKAEFAAP STDAPDKGYV VPNVDLPPLC SSRFRSGPPG EEAQVASQFI ADVIENSQII Q KEDLCISP GKLVWVLYCD LICLDYDGNI LDACTFALLA ALKNVQLPEV ...String:
MAAGFKTVEP LEYYRRFLKE NCRPDGRELG EFRTTTVNIG SISTADGSAL VKLGNTTVIC GVKAEFAAP STDAPDKGYV VPNVDLPPLC SSRFRSGPPG EEAQVASQFI ADVIENSQII Q KEDLCISP GKLVWVLYCD LICLDYDGNI LDACTFALLA ALKNVQLPEV TINEETALAE VN LKKKSYL NIRTHPVATS FAVFDDTLLI VDPTGEEEHL ATGTLTIVMD EEGKLCCLHK PGG SGLTGA KLQDCMSRAV TRHKEVKKLM DEVIKSMKPK

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Macromolecule #4: EXOSC5

MacromoleculeName: EXOSC5 / type: protein_or_peptide / ID: 4 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MEEETHTDAK IRAENGTGSS PRGPGCSLRH FACEQNLLSR PDGSASFLQG DTSVLAGVYG PAEVKVSKE IFNKATLEVI LRPKIGLPGV AEKSRERLIR NTCEAVVLGT LHPRTSITVV L QVVSDAGS LLACCLNAAC MALVDAGVPM RALFCGVACA LDSDGTLVLD ...String:
MEEETHTDAK IRAENGTGSS PRGPGCSLRH FACEQNLLSR PDGSASFLQG DTSVLAGVYG PAEVKVSKE IFNKATLEVI LRPKIGLPGV AEKSRERLIR NTCEAVVLGT LHPRTSITVV L QVVSDAGS LLACCLNAAC MALVDAGVPM RALFCGVACA LDSDGTLVLD PTSKQEKEAR AV LTFALDS VERKLLMSST KGLYSDTELQ QCLAAAQAAS QHVFRFYRES LQRRYSKS

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Macromolecule #5: EXOSC7

MacromoleculeName: EXOSC7 / type: protein_or_peptide / ID: 5 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MASVTLSEAE KVYIVHGVQE DLRVDGRGCE DYRCVEVETD VVSNTSGSAR VKLGHTDILV GVKAEMGTP KLEKPNEGYL EFFVDCSASA TPEFEGRGGD DLGTEIANTL YRIFNNKSSV D LKTLCISP REHCWVLYVD VLLLECGGNL FDAISIAVKA ALFNTRIPRV ...String:
MASVTLSEAE KVYIVHGVQE DLRVDGRGCE DYRCVEVETD VVSNTSGSAR VKLGHTDILV GVKAEMGTP KLEKPNEGYL EFFVDCSASA TPEFEGRGGD DLGTEIANTL YRIFNNKSSV D LKTLCISP REHCWVLYVD VLLLECGGNL FDAISIAVKA ALFNTRIPRV RVLEDEEGSK DI ELSDDPY DCIRLSVENV PCIVTLCKIG YRHVVDATLQ EEACSLASLL VSVTSKGVVT CMR KVGKGS LDPESIFEMM ETGKRVGKVL HASLQSVVHK EESLGPKRQK VGFLG

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Macromolecule #6: EXOSC6

MacromoleculeName: EXOSC6 / type: protein_or_peptide / ID: 6 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MPGDHRRIRG PEESQPPQLY AADEEEAPGT RDPTRLRPVY ARAGLLSQAK GSAYLEAGGT KVLCAVSGP RQAEGGERGG GPAGAGGEAP AALRGRLLCD FRRAPFAGRR RRAPPGGCEE R ELALALQE ALEPAVRLGR YPRAQLEVSA LLLEDGGSAL AAALTAAALA ...String:
MPGDHRRIRG PEESQPPQLY AADEEEAPGT RDPTRLRPVY ARAGLLSQAK GSAYLEAGGT KVLCAVSGP RQAEGGERGG GPAGAGGEAP AALRGRLLCD FRRAPFAGRR RRAPPGGCEE R ELALALQE ALEPAVRLGR YPRAQLEVSA LLLEDGGSAL AAALTAAALA LADAGVEMYD LV VGCGLSL APGPAPTWLL DPTRLEEERA AAGLTVALMP VLNQVAGLLG SGEGGLTESW AEA VRLGLE GCQRLYPVLQ QSLVRAARRR GAAAQP

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Macromolecule #7: EXOSC3

MacromoleculeName: EXOSC3 / type: protein_or_peptide / ID: 7 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAEPASVAAE SLAGSRARAA RTVLGQVVLP GEELL LPEQ EDAEGPGGAV ERPLSLNARA CSRVRVVCGP GLRRCGDRLL VTKCGRLRHK EPGSGS GGG VYWVDSQQKR YVPVKGDHVI GIVTAKSGDI FKVDVGGSEP ASLSYLSFEG ATKRNRP NV QVGDLIYGQF ...String:
MAEPASVAAE SLAGSRARAA RTVLGQVVLP GEELL LPEQ EDAEGPGGAV ERPLSLNARA CSRVRVVCGP GLRRCGDRLL VTKCGRLRHK EPGSGS GGG VYWVDSQQKR YVPVKGDHVI GIVTAKSGDI FKVDVGGSEP ASLSYLSFEG ATKRNRP NV QVGDLIYGQF VVANKDMEPE MVCIDSCGRA NGMGVIGQDG LLFKVTLGLI RKLLAPDC E IIQEVGKLYP LEIVFGMNGR IWVKAKTIQQ TLILANILEA CEHMTSDQRK QIFSRLAES

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Macromolecule #8: EXOSC2

MacromoleculeName: EXOSC2 / type: protein_or_peptide / ID: 8 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAMEMRLPVA RKPLSERLGR DTKKHLVVPG DTITT DTGF MRGHGTYMGE EKLIASVAGS VERVNKLICV KALKTRYIGE VGDIVVGRIT EVQQKR WKV ETNSRLDSVL LLSSMNLPGG ELRRRSAEDE LAMRGFLQEG DLISAEVQAV FSDGAVS LH TRSLKYGKLG ...String:
MAMEMRLPVA RKPLSERLGR DTKKHLVVPG DTITT DTGF MRGHGTYMGE EKLIASVAGS VERVNKLICV KALKTRYIGE VGDIVVGRIT EVQQKR WKV ETNSRLDSVL LLSSMNLPGG ELRRRSAEDE LAMRGFLQEG DLISAEVQAV FSDGAVS LH TRSLKYGKLG QGVLVQVSPS LVKRQKTHFH DLPCGASVIL GNNGFIWIYP TPEHKEEE A GGFIANLEPV SLADREVISR LRNCIISLVT QRMMLYDTSI LYCYEASLPH QIKDILKPE IMEEIVMETR QRLLEQEG

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Macromolecule #9: EXOSC1

MacromoleculeName: EXOSC1 / type: protein_or_peptide / ID: 9 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAPPVRYCIP GERLCNLEEG SPGSGTYTRH GYIFSSLAGC LMKSSENGAL PVVSVVRETE SQLLPDVGA IVTCKVSSIN SRFAKVHILY VGSMPLKNSF RGTIRKEDVR ATEKDKVEIY K SFRPGDIV LAKVISLGDA QSNYLLTTAE NELGVVVAHS ESGIQMVPIS ...String:
MAPPVRYCIP GERLCNLEEG SPGSGTYTRH GYIFSSLAGC LMKSSENGAL PVVSVVRETE SQLLPDVGA IVTCKVSSIN SRFAKVHILY VGSMPLKNSF RGTIRKEDVR ATEKDKVEIY K SFRPGDIV LAKVISLGDA QSNYLLTTAE NELGVVVAHS ESGIQMVPIS WCEMQCPKTH TK EFRKVAR VQPEFLQT

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Macromolecule #10: DIS3

MacromoleculeName: DIS3 / type: protein_or_peptide / ID: 10 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MLKSKTFLKK TRAGGVMKIV REHYLRDDIG CGAPGCAACG GAHEGPALEP QPQDPASSVC PQPHYLLPD TNVLLHQIDV LEDPAIRNVI VLQTVLQEVR NRSAPVYKRI RDVTNNQEKH F YTFTNEHH RETYVEQEQG ENANDRNNRA IRVAAKWYNE HLKKMSADNQ ...String:
MLKSKTFLKK TRAGGVMKIV REHYLRDDIG CGAPGCAACG GAHEGPALEP QPQDPASSVC PQPHYLLPD TNVLLHQIDV LEDPAIRNVI VLQTVLQEVR NRSAPVYKRI RDVTNNQEKH F YTFTNEHH RETYVEQEQG ENANDRNNRA IRVAAKWYNE HLKKMSADNQ LQVIFITNDR RN KEKAIEE GIPAFTCEEY VKSLTANPEL IDRLACLSEE GNEIESGKII FSEHLPLSKL QQG IKSGTY LQGTFRASRE NYLEATVWIH GDNEENKEII LQGLKHLNRA VHEDIVAVEL LPKS QWVAP SSVVLHDEGQ NEEDVEKEEE TERMLKTAVS EKMLKPTGRV VGIIKRNWRP YCGML SKSD IKESRRHLFT PADKRIPRIR IETRQASTLE GRRIIVAIDG WPRNSRYPNG HFVRNL GDV GEKETETEVL LLEHDVPHQP FSQAVLSFLP KMPWSITEKD MKNREDLRHL CICSVDP PG CTDINDALHC RELENGNLEV GVHIADVSHF IRPGNALDQE SARRGTTVYL CEKRIDMV P ELLSSNLCSL KCDVDRLAFS CIWEMNHNAE ILKTKFTKSV INSKASLTYA EAQLRIDSA NMNDDITTSL RGLNKLAKIL KKRRIEKGAL TLSSPEVRFH MDSETHDPID LQTKELRETN SMVEEFMLL ANISVAKKIH EEFSEHALLR KHPAPPPSNY EILVKAARSR NLEIKTDTAK S LAESLDQA ESPTFPYLNT LLRILATRCM MQAVYFCSGM DNDFHHYGLA SPIYTHFTSP IR RYADVIV HRLLAVAIGA DCTYPELTDK HKLADICKNL NFRHKMAQYA QRASVAFHTQ LFF KSKGIV SEEAYILFVR KNAIVVLIPK YGLEGTVFFE EKDKPNPQLI YDDEIPSLKI EDTV FHVFD KVKVKIMLDS SNLQHQKIRM SLVEPQIPGI SIPTDTSNMD LNGPKKKKMK LGK

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Macromolecule #11: MPP6

MacromoleculeName: MPP6 / type: protein_or_peptide / ID: 11 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
ASMAAERKTK LSKNLLRMKF MQRGLDSETK KQLEE EEKK IISEEHWYLD LPELKEKESF IIEEQSFLLC EDLLYGRMSF RGFNPEVEKL MLQMNA KHK AEEVEDETVE LDVSDEEMAR RYETLVGTIG KKFARKRDHA NYEEDENGDI TPIKAKK MF LKPQD

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Macromolecule #12: EXOSC10

MacromoleculeName: EXOSC10 / type: protein_or_peptide / ID: 12 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MAPPSTREPR VLSATSATKS DGEMVLPGFP DADSFVKFAL GSVVAVTKAS GGLPQFGDEY DFYRSFPGF QAFCETQGDR LLQCMSRVMQ YHGCRSNIKD RSKVTELEDK FDLLVDANDV I LERVGILL DEASGVNKNQ QPVLPAGLQV PKTVVSSWNR KAAEYGKKAK ...String:
MAPPSTREPR VLSATSATKS DGEMVLPGFP DADSFVKFAL GSVVAVTKAS GGLPQFGDEY DFYRSFPGF QAFCETQGDR LLQCMSRVMQ YHGCRSNIKD RSKVTELEDK FDLLVDANDV I LERVGILL DEASGVNKNQ QPVLPAGLQV PKTVVSSWNR KAAEYGKKAK SETFRLLHAK NI IRPQLKF REKIDNSNTP FLPKIFIKPN AQKPLPQALS KERRERPQDR PEDLDVPPAL ADF IHQQRT QQVEQDMFAH PYQYELNHFT PADAVLQKPQ PQLYRPIEET PCHFISSLDE LVEL NEKLL NCQEFAVDLE HHSYRSFLGL TCLMQISTRT EDFIIDTLEL RSDMYILNES LTDPA IVKV FHGADSDIEW LQKDFGLYVV NMFDTHQAAR LLNLGRHSLD HLLKLYCNVD SNKQYQ LAD WRIRPLPEEM LSYARDDTHY LLYIYDKMRL EMWERGNGQP VQLQVVWQRS RDICLKK FI KPIFTDESYL ELYRKQKKHL NTQQLTAFQL LFAWRDKTAR REDESYGYVL PNHMMLKI A EELPKEPQGI IACCNPVPPL VRQQINEMHL LIQQAREMPL LKSEVAAGVK KSGPLPSAE RLENVLFGPH DCSHAPPDGY PIIPTSGSVP VQKQASLFPD EKEDNLLGTT CLIATAVITL FNEPSAEDS KKGPLTVAQK KAQNIMESFE NPFRMFLPSL GHRAPVSQAA KFDPSTKIYE I SNRWKLAQ VQVQKDSKEA VKKKAAEQTA AREQAKEACK AAAEQAISVR QQVVLENAAK KR ERATSDP RTTEQKQEKK RLKISKKPKD PEPPEKEFTP YDYSQSDFKA FAGNSKSKVS SQF DPNKQT PSGKKCIAAK KIKQSVGNKS MSFPTGKSDR GFRYNWPQR

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Macromolecule #13: C1D

MacromoleculeName: C1D / type: protein_or_peptide / ID: 13 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString:
MAGEEINEDY PVEIHEYLSA FENSIGAVDE MLKTMMSVSR NELLQKLDPL EQAKVDLVSA YTLNSMFWV YLATQGVNPK EHPVKQELER IRVYMNRVKE ITDKKKAGKL DRGAASRFVK N ALWEPKSK NASKVANKGK SKS

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Macromolecule #14: MTR4

MacromoleculeName: MTR4 / type: protein_or_peptide / ID: 14 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Recombinant expressionOrganism: Escherichia coli (E. coli)
SequenceString: MADAFGDELF SVFEGDSTTA AGTKKDKEKD KGKWKGPPGS ADKAGKRFDG KLQSESTNNG KNKRDVDFE GTDEPIFGKK PRIEESITED LSLADLMPRV KVQSVETVEG CTHEVALPAE E DYLPLKPR VGKAAKEYPF ILDAFQREAI QCVDNNQSVL VSAHTSAGKT ...String:
MADAFGDELF SVFEGDSTTA AGTKKDKEKD KGKWKGPPGS ADKAGKRFDG KLQSESTNNG KNKRDVDFE GTDEPIFGKK PRIEESITED LSLADLMPRV KVQSVETVEG CTHEVALPAE E DYLPLKPR VGKAAKEYPF ILDAFQREAI QCVDNNQSVL VSAHTSAGKT VCAEYAIALA LR EKQRVIF TSPIKALSNQ KYREMYEEFQ DVGLMTGDVT INPTASCLVM TTEILRSMLY RGS EVMREV AWVIFDEIHY MRDSERGVVW EETIILLPDN VHYVFLSATI PNARQFAEWI CHLH KQPCH VIYTDYRPTP LQHYIFPAGG DGLHLVVDEN GDFREDNFNT AMQVLRDAGD LAKGD QKGR KGGTKGPSNV FKIVKMIMER NFQPVIIFSF SKKDCEAYAL QMTKLDFNTD EEKKMV EEV FSNAIDCLSD EDKKLPQVEH VLPLLKRGIG IHHGGLLPIL KETIEILFSE GLIKALF AT ETFAMGINMP ARTVLFTNAR KFDGKDFRWI SSGEYIQMSG RAGRRGMDDR GIVILMVD E KMSPTIGKQL LKGSADPLNS AFHLTYNMVL NLLRVEEINP EYMLEKSFYQ FQHYRAIPG VVEKVKNSEE QYNKIVIPNE ESVVIYYKIR QQLAKLGKEI EEYIHKPKYC LPFLQPGRLV KVKNEGDDF GWGVVVNFSK KSNVKPNSGE LDPLYVVEVL LRCSKESLKN SATEAAKPAK P DEKGEMQV VPVLVHLLSA ISSVRLYIPK DLRPVDNRQS VLKSIQEVQK RFPDGIPLLD PI DDMGIQD QGLKKVIQKV EAFEHRMYSH PLHNDPNLET VYTLCEKKAQ IAIDIKSAKR ELK KARTVL QMDELKCRKR VLRRLGFATS SDVIEMKGRV ACEISSADEL LLTEMMFNGL FNDL SAEQA TALLSCFVFQ ENSSEMPKLT EQLAGPLRQM QECAKRIAKV SAEAKLEIDE ETYLS SFKP HLMDVVYTWA TGATFAHICK MTDVFEGSII RCMRRLEELL RQMCQAAKAI GNTELE NKF AEGITKIKRD IVFAASLYL

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Macromolecule #15: U44 ssRNA

MacromoleculeName: U44 ssRNA / type: dna / ID: 15 / Classification: DNA
Source (natural)Organism: synthetic construct (others)
SequenceString:
UUUUUUUUUU UUUUUUUUUU UUUUUUUUUU UUUUUUUUUU UUUU

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.3 mg/mL
BufferpH: 7.5
Component:
ConcentrationName
20.0 mMHepes
150.0 mMNaCl
2.0 mMDTT
VitrificationCryogen name: ETHANE-PROPANE / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Number real images: 8047 / Average electron dose: 47.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 2396236
CTF correctionSoftware - Name: Gctf
Final reconstructionResolution.type: BY AUTHOR / Resolution: 6.25 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 2.1) / Number images used: 13804
Initial angle assignmentType: PROJECTION MATCHING
Final angle assignmentType: PROJECTION MATCHING

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