6KNM

Apelin receptor in complex with single domain antibody

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (B)	Apelin receptor,Rubredoxin,Apelin receptor	polymer	407	46443.6	1	UniProt (P35414) UniProt (P00268) Pfam (PF00001) Pfam (PF00301)	Homo sapiens (Human)	Angiotensin receptor-like 1,G-protein coupled receptor APJ,G-protein coupled receptor HG11,Rd,Angiotensin receptor-like 1,G-protein coupled receptor APJ,G-protein coupled receptor HG11
2	B (A)	Single domain antibody JN241	polymer	129	13766.3	1		Camelus bactrianus
3	C (B)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)

Sequence modifications

B: 7 - 229 (UniProt: P35414)

PDB	External Database	Details
Met -17	-	initiating methionine
Lys -16	-	expression tag
Thr -15	-	expression tag
Ile -14	-	expression tag
Ile -13	-	expression tag
Ala -12	-	expression tag
Leu -11	-	expression tag
Ser -10	-	expression tag
Tyr -9	-	expression tag
Ile -8	-	expression tag
Phe -7	-	expression tag
Cys -6	-	expression tag
Leu -5	-	expression tag
Val -4	-	expression tag
Phe -3	-	expression tag
Ala -2	-	expression tag
Asp -1	-	expression tag
Tyr 0	-	expression tag
Lys 1	-	expression tag
Asp 2	-	expression tag
Asp 3	-	expression tag
Asp 4	-	expression tag
Asp 5	-	expression tag
Lys 6	-	expression tag
Ala 117	Val 117	engineered mutation
Asn 177	Thr 177	engineered mutation

B: 1001 - 1054 (UniProt: P00268)
B: 243 - 330 (UniProt: P35414)

PDB	External Database	Details
Lys 261	Trp 261	engineered mutation
Leu 325	Cys 325	engineered mutation
Met 326	Cys 326	engineered mutation
Leu 331	-	expression tag
Glu 332	-	expression tag
Val 333	-	expression tag
Leu 334	-	expression tag
Phe 335	-	expression tag
Gln 336	-	expression tag
Gly 337	-	expression tag
Pro 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag
His 344	-	expression tag
His 345	-	expression tag
His 346	-	expression tag
His 347	-	expression tag
His 348	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	60209.9
Non-Polymers*	Number of molecules	1
	Total formula weight	65.4
All*	Total formula weight	60275.4

*Water molecules are not included.