6KMR

Crystal structure of human N6amt1-Trm112 in complex with SAM (space group P6122)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Multifunctional methyltransferase subunit TRM112-like protein	polymer	126	14346.6	1	UniProt (Q9UI30) Pfam (PF03966)	Homo sapiens (Human)	tRNA methyltransferase 112 homolog
2	B (B)	Methyltransferase N6AMT1	polymer	228	24597.1	1	UniProt (Q9Y5N5) Pfam (PF05175)	Homo sapiens (Human)	HemK methyltransferase family member 2,M.HsaHemK2P,Methylarsonite methyltransferase N6AMT1,N(6)-adenine-specific DNA methyltransferase 1,Protein N(5)-glutamine methyltransferase
3	C (A)	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
4	D (B)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
5	E, F (A, B)	water	water		18.0	192	Chemie (HOH)

Sequence modifications

A: 1 - 125 (UniProt: Q9UI30)

PDB	External Database	Details
Met 0	-	initiating methionine

B: 1 - 214 (UniProt: Q9Y5N5)

PDB	External Database	Details
Met -13	-	expression tag
Gly -12	-	expression tag
Ser -11	-	expression tag
Ser -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
Ser -3	-	expression tag
Gln -2	-	expression tag
Asp -1	-	expression tag
Pro 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	38943.6
Non-Polymers*	Number of molecules	2
	Total formula weight	460.5
All*	Total formula weight	39404.1

*Water molecules are not included.