6KMR

Crystal structure of human N6amt1-Trm112 in complex with SAM (space group P6122)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-25
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.978
Spacegroup name	P 61 2 2
Unit cell lengths	109.708, 109.708, 130.786
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.026 - 2.000
R-factor	0.1632
Rwork	0.162
R-free	0.19180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6kms
RMSD bond length	0.010
RMSD bond angle	1.128
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.115	0.073	0.608
Rmeas	0.125	0.080	0.661
Rpim	0.049	0.031	0.254
Total number of observations	206280
Number of reflections	31971	3454	3126
<I/σ(I)>	4.3
Completeness [%]	99.7	99.3	99.6
Redundancy	6.5	6.2	6.5
CC(1/2)		0.993	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	0.1M succinic acid, pH 7.0, 15% PEG 3350