6KDN

HIV-1 reverse transcriptase with Q151M/Y115F/F116Y:DNA:dGTP ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C	HIV-1 reverse transcriptase p66 subunit	polymer	557	64033.4	2	UniProt (D3XFN5) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) Pfam (PF00075) UniProt (by SIFTS) (P12497) In PDB	Human immunodeficiency virus 1
2	B, D	HIV-1 RT p51 subunit	polymer	444	51861.5	2	UniProt (P12497) Pfam (PF00078) Pfam (PF06817) Pfam (PF06815) In PDB	Human immunodeficiency virus type 1	HIV-1 reverse transcriptase p51 subunit, p51 RT
3	E, F	DNA/RNA (38-MER)	polymer	38	11724.5	2		synthetic construct
4	A, C	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	A, C	2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (DGT)
6	A, B, D	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
7		water	water		18.0	442	Chemie (HOH)

Sequence modifications

A, C: 1 - 555 (UniProt: D3XFN5)

PDB	External Database	Details
Met -1	-	expression tag
Val 0	-	expression tag
Phe 115	Tyr 214	engineered mutation
Tyr 116	Phe 215	engineered mutation
Met 151	Gln 250	engineered mutation
Ser 162	Cys 261	engineered mutation
Ser 280	Cys 379	engineered mutation

B, D: 1 - 428 (UniProt: P12497)

PDB	External Database	Details
Met -15	-	expression tag
Ala -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ala -7	-	expression tag
Leu -6	-	expression tag
Glu -5	-	expression tag
Val -4	-	expression tag
Leu -3	-	expression tag
Phe -2	-	expression tag
Gln -1	-	expression tag
Gly 0	-	expression tag
Ser 162	Cys 749	engineered mutation
Ser 280	Cys 867	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	255238.9
Non-Polymers*	Number of molecules	9
	Total formula weight	1523.4
All*	Total formula weight	256762.3

*Water molecules are not included.