6JE9

Crystal structure of Nme1Cas9-sgRNA dimer mediated by double protein inhibitor AcrIIC3 monomers

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	CRISPR-associated endonuclease Cas9	polymer	1092	125935.2	2	UniProt (C9X1G5) Pfam (PF13395) Pfam (PF18541)	Neisseria meningitidis 8013
2	B, D (B, D)	sgRNA	polymer	135	43059.4	2		synthetic construct
3	E, F (E, F)	AcrIIC3	polymer	117	13457.4	2	UniProt (A0A3E2QDI5) UniProt (by SIFTS) (A0A425B395)	Neisseria meningitidis 8013

Sequence modifications

A, C: 1 - 1082 (UniProt: C9X1G5)

PDB	External Database	Details
Ser 1083	-	expression tag
Glu 1084	-	expression tag
His 1085	-	expression tag
His 1086	-	expression tag
His 1087	-	expression tag
His 1088	-	expression tag
His 1089	-	expression tag
His 1090	-	expression tag
His 1091	-	expression tag
His 1092	-	expression tag

E, F: 1 - 116 (UniProt: A0A3E2QDI5)

PDB	External Database	Details
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	364903.8
All*	Total formula weight	364903.8

*Water molecules are not included.