6IY9

Crystal structure of aminoglycoside 7"-phoshotransferase-Ia (APH(7")-Ia/HYG) from Streptomyces hygroscopicus complexed with hygromycin B

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Hygromycin-B 7''-O-kinase	polymer	340	38292.3	2	UniProt (P09979) Pfam (PF01636) In PDB	Streptomyces hygroscopicus	Aminoglycoside phosphotransferase 7"-Ia, APH(7''),Hygromycin B phosphotransferase,Hygromycin-B kinase
2	A, B	HYGROMYCIN B VARIANT	non-polymer		527.5	2	Chemie (HY0)
3	A, B	2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID	non-polymer		207.3	2	Chemie (NHE)
4	A, B	CITRATE ANION	non-polymer		189.1	2	Chemie (FLC)
5		water	water		18.0	21	Chemie (HOH)

Sequence modifications

A, B: 1 - 332 (UniProt: P09979)

PDB	External Database	Details
Gly 63	Asp 63	engineered mutation
Leu 333	-	expression tag
Glu 334	-	expression tag
His 335	-	expression tag
His 336	-	expression tag
His 337	-	expression tag
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76584.6
Non-Polymers*	Number of molecules	6
	Total formula weight	1847.8
All*	Total formula weight	78432.5

*Water molecules are not included.