6IY9

Crystal structure of aminoglycoside 7"-phoshotransferase-Ia (APH(7")-Ia/HYG) from Streptomyces hygroscopicus complexed with hygromycin B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	PIXEL
Collection date	2018-02-04
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97934
Spacegroup name	P 2 3
Unit cell lengths	151.590, 151.590, 151.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.900 - 2.400
R-factor	0.226
Rwork	0.225
R-free	0.25100
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	PHENIX (1.15.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.132
Number of reflections	45592	6572
<I/σ(I)>	31.2	1.1
Completeness [%]	100.0	100
Redundancy	62.7	64.6
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	0.1 M CHES, pH 8.8, 1.38-1.40 M sodium citrate tribasic