6IY6

Crystal structure of human cytosolic aspartyl-tRNA synthetase (DRS) in complex with glutathion-S transferase (GST) domains from Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 (AIMP2) and glutamyl-prolyl-tRNA synthetase (EPRS)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, G, H	Aspartate--tRNA ligase, cytoplasmic	polymer	502	57310.1	4	UniProt (P14868) Pfam (PF01336) Pfam (PF00152) In PDB	Homo sapiens (Human)	Aspartyl-tRNA synthetase,AspRS,Cell proliferation-inducing gene 40 protein
2	C, D, I, J	Aminoacyl tRNA synthase complex-interacting multifunctional protein 2	polymer	215	24165.0	4	UniProt (Q13155) Pfam (PF18569) Pfam (PF00043) In PDB	Homo sapiens (Human)	Multisynthase complex auxiliary component p38,Protein JTV-1
3	E, F, K, L	Bifunctional glutamate/proline--tRNA ligase	polymer	165	18434.8	4	UniProt (P07814) Pfam (PF21972) In PDB	Homo sapiens (Human)	Bifunctional aminoacyl-tRNA synthetase,Cell proliferation-inducing gene 32 protein,Glutamatyl-prolyl-tRNA synthetase
4	H, A	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	G, H, A, B	PHOSPHATE ION	non-polymer		95.0	17	Chemie (PO4)
6		water	water		18.0	12	Chemie (HOH)

Sequence modifications

A, B, G, H: 21 - 501 (UniProt: P14868)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	expression tag
Ser 2	-	expression tag
Ser 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
Ser 10	-	expression tag
Ser 11	-	expression tag
Gly 12	-	expression tag
Leu 13	-	expression tag
Val 14	-	expression tag
Pro 15	-	expression tag
Arg 16	-	expression tag
Gly 17	-	expression tag
Ser 18	-	expression tag
His 19	-	expression tag
Met 20	-	expression tag

C, D, I, J: 115 - 320 (UniProt: Q13155)

PDB	External Database	Details
Met 114	-	initiating methionine
Leu 321	-	expression tag
Glu 322	-	expression tag
His 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag

E, F, K, L: 1 - 157 (UniProt: P07814)

PDB	External Database	Details
Leu 158	-	expression tag
Glu 159	-	expression tag
His 160	-	expression tag
His 161	-	expression tag
His 162	-	expression tag
His 163	-	expression tag
His 164	-	expression tag
His 165	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	399639.8
Non-Polymers*	Number of molecules	19
	Total formula weight	1745.3
All*	Total formula weight	401385.1

*Water molecules are not included.