6IY6

Crystal structure of human cytosolic aspartyl-tRNA synthetase (DRS) in complex with glutathion-S transferase (GST) domains from Aminoacyl tRNA synthase complex-interacting multifunctional protein 2 (AIMP2) and glutamyl-prolyl-tRNA synthetase (EPRS)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-24
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9793
Spacegroup name	P 61
Unit cell lengths	108.068, 108.068, 815.642
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 3.600
R-factor	0.2386
Rwork	0.237
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4j15 5a34
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.660
High resolution limit [Å]	3.600	3.600
Number of reflections	61327	2998
<I/σ(I)>	8.18
Completeness [%]	98.7
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	295	0.1 M CAPS (pH 10.5) 1.3 M sodium phosphate monobasic 0.5 M potassium phosphate dibasic 0.5 M lithium sulfate