6IM9

MDM2 bound CueO-PM2 sensor

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Blue copper oxidase CueO,PM2 peptide,Blue copper oxidase CueO	polymer	509	55846.8	1	UniProt (P36649) Pfam (PF07732) Pfam (PF00394) Pfam (PF07731) In PDB	Escherichia coli (strain K12)	Copper efflux oxidase
2	B	E3 ubiquitin-protein ligase Mdm2	polymer	122	13749.7	1	UniProt (Q00987) Pfam (PF02201) In PDB	Homo sapiens (Human)	Double minute 2 protein,Hdm2,Oncoprotein Mdm2,RING-type E3 ubiquitin transferase Mdm2,p53-binding protein Mdm2

Sequence modifications

A: 29 - 384 (UniProt: P36649)

PDB	External Database	Details
Ile 358	Met 358	engineered mutation

A: 385 - 395 (PDB: 6IM9)
A: 396 - 516 (UniProt: P36649)

PDB	External Database	Details
Lys 517	-	expression tag
Asp 518	-	expression tag
Pro 519	-	expression tag
Asn 520	-	expression tag
Ser 521	-	expression tag
Ser 522	-	expression tag
Ser 523	-	expression tag
Val 524	-	expression tag
Asp 525	-	expression tag
Lys 526	-	expression tag
Leu 527	-	expression tag
Ala 528	-	expression tag
Ala 529	-	expression tag
Ala 530	-	expression tag
Leu 531	-	expression tag
Glu 532	-	expression tag
His 533	-	expression tag
His 534	-	expression tag
His 535	-	expression tag
His 536	-	expression tag
His 537	-	expression tag

B: 6 - 125 (UniProt: Q00987)

PDB	External Database	Details
Gly 4	-	expression tag
Pro 5	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	69596.5
All*	Total formula weight	69596.5

*Water molecules are not included.