6IM9
MDM2 bound CueO-PM2 sensor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 83.636, 83.636, 240.171 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.920 - 3.300 |
| R-factor | 0.1801 |
| Rwork | 0.177 |
| R-free | 0.24930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1KV7 and 5WTS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.215 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.380 |
| High resolution limit [Å] | 3.300 | 8.090 | 3.300 |
| Rmerge | 0.046 | 0.019 | 0.282 |
| Rmeas | 0.052 | 0.021 | 0.326 |
| Rpim | 0.023 | 0.009 | 0.159 |
| Total number of observations | 77012 | ||
| Number of reflections | 15209 | 1129 | 850 |
| <I/σ(I)> | 14.1 | ||
| Completeness [%] | 98.7 | 98.8 | 85.8 |
| Redundancy | 5.1 | 4.8 | 3.4 |
| CC(1/2) | 0.999 | 0.961 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 4M sodium formate |
