6ILH

Crystal Structure of human lysyl-tRNA synthetase L350H mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Lysine-tRNA ligase	polymer	521	60336.3	2	UniProt (Q15046) Pfam (PF01336) Pfam (PF00152)	Homo sapiens (Human)	Lysyl-tRNA synthetase,LysRS
2	C, D (A, B)	5'-O-[(L-LYSYLAMINO)SULFONYL]ADENOSINE	non-polymer		474.5	2	Chemie (KAA)
3	E, F (A, B)	water	water		18.0	53	Chemie (HOH)

Sequence modifications

A, B: 70 - 581 (UniProt: Q15046)

PDB	External Database	Details
Met 69	-	initiating methionine
His 350	Leu 350	engineered mutation
Leu 582	-	expression tag
Glu 583	-	expression tag
His 584	-	expression tag
His 585	-	expression tag
His 586	-	expression tag
His 587	-	expression tag
His 588	-	expression tag
His 589	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	120672.6
Non-Polymers*	Number of molecules	2
	Total formula weight	949.0
All*	Total formula weight	121621.6

*Water molecules are not included.