6I3F

Crystal structure of the complex of human angiotensinogen and renin at 2.55 Angstrom

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Angiotensinogen	polymer	458	50647.7	1	UniProt (P01019) Pfam (PF00079)	Homo sapiens (Human)	Serpin A8
2	B (B)	Renin	polymer	340	37223.0	1	UniProt (P00797) Pfam (PF00026)	Homo sapiens (Human)	Angiotensinogenase
3	C (C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-2)-alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		951.9	1	In PDB GlyTouCan (G10133VD)
4	D (D)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose	branched		570.5	1	In PDB GlyTouCan (G21290RB)
5	E, F, H, I, J... (A, B)	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)
6	G (A)	GLYCEROL	non-polymer		92.1	1	Chemie (GOL)
7	P, Q (A, B)	water	water		18.0	170	Chemie (HOH)

Sequence modifications

A: 1 - 452 (UniProt: P01019)

PDB	External Database	Details
Gln 137	Asn 170	engineered mutation
Ser 232	Cys 265	engineered mutation
Gln 271	Asn 304	engineered mutation
Gln 295	Asn 328	engineered mutation
Ser 308	Cys 341	engineered mutation
His 453	-	expression tag
His 454	-	expression tag
His 455	-	expression tag
His 456	-	expression tag
His 457	-	expression tag
His 458	-	expression tag

B: 1 - 340 (UniProt: P00797)

PDB	External Database	Details
Ala 226	Asp 292	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	87870.7
Branched	Number of molecules	2
	Total formula weight	1522.4
Non-Polymers*	Number of molecules	11
	Total formula weight	1052.7
All*	Total formula weight	90445.8

*Water molecules are not included.