6HUJ
CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with picrotoxin, GABA and megabody Mb38.
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, D (A, D) | Gamma-aminobutyric acid receptor subunit alpha-1,Gamma-aminobutyric acid receptor subunit alpha-1 | polymer | 464 | 52916.6 | 2 | UniProt (P08219) UniProt (P14867) Pfam (PF02931) Pfam (PF02932) | Bos taurus (Bovine) | GABA(A) receptor subunit alpha-1,GABA(A) receptor subunit alpha-1 |
| 2 | B, E (B, E) | Gamma-aminobutyric acid receptor subunit beta-3 | polymer | 473 | 54444.6 | 2 | UniProt (P28472) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | GABA(A) receptor subunit beta-3 |
| 3 | C (C) | Gamma-aminobutyric acid receptor subunit gamma-2 | polymer | 495 | 56922.1 | 1 | UniProt (P18507) Pfam (PF02931) Pfam (PF02932) | Homo sapiens (Human) | GABA(A) receptor subunit gamma-2 |
| 4 | F (G) | Megabody Mb38 | polymer | 539 | 57784.3 | 1 | Lama glama | ||
| 5 | G (F) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1235.1 | 1 | In PDB GlyTouCan (G55220VL) | |||
| 6 | H, M (H, M) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose-(1-6)-[alpha-D-mannopyranose-(1-3)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 1073.0 | 2 | In PDB GlyTouCan (G09724ZC) | |||
| 7 | I, J, L (I, J, L) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 3 | In PDB GlyTouCan (G42666HT) | |||
| 8 | K (K) | alpha-D-mannopyranose-(1-3)-[alpha-D-mannopyranose-(1-6)]beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 910.8 | 1 | In PDB GlyTouCan (G22768VO) | |||
| 9 | N, Q (A, D) | [(2R)-2-octanoyloxy-3-[oxidanyl-[(1R,2R,3S,4R,5R,6S)-2,3,6-tris(oxidanyl)-4,5-diphosphonooxy-cyclohexyl]oxy-phosphoryl]oxy-propyl] octanoate | non-polymer | 746.6 | 2 | Chemie (PIO) PubChem (16219852) PubChem (52946598) PubChem (49867504) PubChem (53394310) PubChem (73441659) PubChem (124587602) PubChem (131850384) PubChem (132274717) PubChem (99649229) PubChem (9543520) PubChem (136212530) PubChem (136212534) | |||
| 10 | O (A) | (1aR,2aR,3S,6R,6aS,8aS,8bR,9R)-2a-hydroxy-8b-methyl-9-(prop-1-en-2-yl)hexahydro-3,6-methano-1,5,7-trioxacyclopenta[ij]c yclopropa[a]azulene-4,8(3H)-dione | non-polymer | 292.3 | 1 | Chemie (RI5) PubChem (16759291) PubChem (17756773) PubChem (4816) PubChem (176519984) PubChem (23305071) PubChem (23307258) PubChem (23425892) PubChem (177503956) PubChem (24041599) PubChem (24893782) PubChem (31306) PubChem (22524996) PubChem (23307236) PubChem (50919219) PubChem (442292) PubChem (71296878) PubChem (73707459) PubChem (73759784) PubChem (73896911) PubChem (689049) PubChem (1268032) PubChem (88590523) PubChem (88909922) PubChem (5320588) PubChem (5458939) PubChem (98140704) PubChem (98140703) PubChem (5702165) PubChem (6419901) PubChem (6604945) PubChem (6978719) PubChem (6604354) PubChem (98140702) PubChem (98140705) PubChem (117072374) PubChem (7098683) PubChem (122130660) PubChem (122130784) PubChem (124080967) PubChem (12314267) PubChem (12314268) PubChem (126456804) PubChem (170924269) PubChem (172433597) PubChem (129700527) PubChem (131673970) PubChem (145948324) PubChem (145948333) PubChem (155929809) PubChem (162968642) PubChem (162968643) PubChem (164802118) PubChem (171378856) | |||
| 11 | P, R (A, D) | GAMMA-AMINO-BUTANOIC ACID | non-polymer | 103.1 | 2 | Chemie (ABU) PubChem (119) |
Sequence modifications
A, D: -34 - -8 (UniProt: P08219)
A, D: 1 - 429 (UniProt: P14867)
C: -38 - 436 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Asp -7 | - | linker |
| Tyr -6 | - | linker |
| Lys -5 | - | linker |
| Asp -4 | - | linker |
| Asp -3 | - | linker |
| Asp -2 | - | linker |
| Asp -1 | - | linker |
| Lys 0 | - | linker |
C: -38 - 436 (UniProt: P18507)
| PDB | External Database | Details |
|---|---|---|
| Gly 437 | - | expression tag |
| Gly 438 | - | expression tag |
| Ser 439 | - | expression tag |
| Gly 440 | - | expression tag |
| Gly 441 | - | expression tag |
| Ser 442 | - | expression tag |
| Gly 443 | - | expression tag |
| Gly 444 | - | expression tag |
| Ser 445 | - | expression tag |
| Gly 446 | - | expression tag |
| Lys 447 | - | expression tag |
| Thr 448 | - | expression tag |
| Glu 449 | - | expression tag |
| Thr 450 | - | expression tag |
| Ser 451 | - | expression tag |
| Gln 452 | - | expression tag |
| Val 453 | - | expression tag |
| Ala 454 | - | expression tag |
| Pro 455 | - | expression tag |
| Ala 456 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 329428.7 | |
| Branched | Number of molecules | 7 |
| Total formula weight | 5565.1 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 1991.7 | |
| All* | Total formula weight | 336985.4 |
