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6HUJ

CryoEM structure of human full-length heteromeric alpha1beta3gamma2L GABA(A)R in complex with picrotoxin, GABA and megabody Mb38.

Functional Information from GO Data
ChainGOidnamespacecontents
A0004888molecular_functiontransmembrane signaling receptor activity
A0004890molecular_functionGABA-A receptor activity
A0005216molecular_functionmonoatomic ion channel activity
A0005230molecular_functionextracellular ligand-gated monoatomic ion channel activity
A0005237molecular_functioninhibitory extracellular ligand-gated monoatomic ion channel activity
A0005254molecular_functionchloride channel activity
A0005886cellular_componentplasma membrane
A0006811biological_processmonoatomic ion transport
A0006821biological_processchloride transport
A0007165biological_processsignal transduction
A0007214biological_processgamma-aminobutyric acid signaling pathway
A0008503molecular_functionbenzodiazepine receptor activity
A0016020cellular_componentmembrane
A0016917molecular_functionGABA receptor activity
A0022851molecular_functionGABA-gated chloride ion channel activity
A0030659cellular_componentcytoplasmic vesicle membrane
A0031410cellular_componentcytoplasmic vesicle
A0032590cellular_componentdendrite membrane
A0034220biological_processmonoatomic ion transmembrane transport
A0034707cellular_componentchloride channel complex
A0043005cellular_componentneuron projection
A0045202cellular_componentsynapse
A0045211cellular_componentpostsynaptic membrane
A0050877biological_processnervous system process
A0051932biological_processsynaptic transmission, GABAergic
A0060078biological_processregulation of postsynaptic membrane potential
A0098794cellular_componentpostsynapse
A0098982cellular_componentGABA-ergic synapse
A1902476biological_processchloride transmembrane transport
A1902495cellular_componenttransmembrane transporter complex
A1902710cellular_componentGABA receptor complex
A1902711cellular_componentGABA-A receptor complex
A1904315molecular_functiontransmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential
A1904862biological_processinhibitory synapse assembly
B0004888molecular_functiontransmembrane signaling receptor activity
B0004890molecular_functionGABA-A receptor activity
B0005216molecular_functionmonoatomic ion channel activity
B0005230molecular_functionextracellular ligand-gated monoatomic ion channel activity
B0005254molecular_functionchloride channel activity
B0005886cellular_componentplasma membrane
B0006811biological_processmonoatomic ion transport
B0006821biological_processchloride transport
B0007165biological_processsignal transduction
B0007214biological_processgamma-aminobutyric acid signaling pathway
B0007268biological_processchemical synaptic transmission
B0016020cellular_componentmembrane
B0022851molecular_functionGABA-gated chloride ion channel activity
B0030594molecular_functionneurotransmitter receptor activity
B0030659cellular_componentcytoplasmic vesicle membrane
B0031410cellular_componentcytoplasmic vesicle
B0034220biological_processmonoatomic ion transmembrane transport
B0034707cellular_componentchloride channel complex
B0042391biological_processregulation of membrane potential
B0042802molecular_functionidentical protein binding
B0043005cellular_componentneuron projection
B0045202cellular_componentsynapse
B0045211cellular_componentpostsynaptic membrane
B0050877biological_processnervous system process
B0051932biological_processsynaptic transmission, GABAergic
B0060021biological_processroof of mouth development
B0071420biological_processcellular response to histamine
B1902476biological_processchloride transmembrane transport
B1902495cellular_componenttransmembrane transporter complex
B1902711cellular_componentGABA-A receptor complex
B1904862biological_processinhibitory synapse assembly
C0004888molecular_functiontransmembrane signaling receptor activity
C0004890molecular_functionGABA-A receptor activity
C0005216molecular_functionmonoatomic ion channel activity
C0005230molecular_functionextracellular ligand-gated monoatomic ion channel activity
C0006811biological_processmonoatomic ion transport
C0006821biological_processchloride transport
C0007214biological_processgamma-aminobutyric acid signaling pathway
C0016020cellular_componentmembrane
C0034220biological_processmonoatomic ion transmembrane transport
D0004888molecular_functiontransmembrane signaling receptor activity
D0004890molecular_functionGABA-A receptor activity
D0005216molecular_functionmonoatomic ion channel activity
D0005230molecular_functionextracellular ligand-gated monoatomic ion channel activity
D0005237molecular_functioninhibitory extracellular ligand-gated monoatomic ion channel activity
D0005254molecular_functionchloride channel activity
D0005886cellular_componentplasma membrane
D0006811biological_processmonoatomic ion transport
D0006821biological_processchloride transport
D0007165biological_processsignal transduction
D0007214biological_processgamma-aminobutyric acid signaling pathway
D0008503molecular_functionbenzodiazepine receptor activity
D0016020cellular_componentmembrane
D0016917molecular_functionGABA receptor activity
D0022851molecular_functionGABA-gated chloride ion channel activity
D0030659cellular_componentcytoplasmic vesicle membrane
D0031410cellular_componentcytoplasmic vesicle
D0032590cellular_componentdendrite membrane
D0034220biological_processmonoatomic ion transmembrane transport
D0034707cellular_componentchloride channel complex
D0043005cellular_componentneuron projection
D0045202cellular_componentsynapse
D0045211cellular_componentpostsynaptic membrane
D0050877biological_processnervous system process
D0051932biological_processsynaptic transmission, GABAergic
D0060078biological_processregulation of postsynaptic membrane potential
D0098794cellular_componentpostsynapse
D0098982cellular_componentGABA-ergic synapse
D1902476biological_processchloride transmembrane transport
D1902495cellular_componenttransmembrane transporter complex
D1902710cellular_componentGABA receptor complex
D1902711cellular_componentGABA-A receptor complex
D1904315molecular_functiontransmitter-gated monoatomic ion channel activity involved in regulation of postsynaptic membrane potential
D1904862biological_processinhibitory synapse assembly
E0004888molecular_functiontransmembrane signaling receptor activity
E0004890molecular_functionGABA-A receptor activity
E0005216molecular_functionmonoatomic ion channel activity
E0005230molecular_functionextracellular ligand-gated monoatomic ion channel activity
E0005254molecular_functionchloride channel activity
E0005886cellular_componentplasma membrane
E0006811biological_processmonoatomic ion transport
E0006821biological_processchloride transport
E0007165biological_processsignal transduction
E0007214biological_processgamma-aminobutyric acid signaling pathway
E0007268biological_processchemical synaptic transmission
E0016020cellular_componentmembrane
E0022851molecular_functionGABA-gated chloride ion channel activity
E0030594molecular_functionneurotransmitter receptor activity
E0030659cellular_componentcytoplasmic vesicle membrane
E0031410cellular_componentcytoplasmic vesicle
E0034220biological_processmonoatomic ion transmembrane transport
E0034707cellular_componentchloride channel complex
E0042391biological_processregulation of membrane potential
E0042802molecular_functionidentical protein binding
E0043005cellular_componentneuron projection
E0045202cellular_componentsynapse
E0045211cellular_componentpostsynaptic membrane
E0050877biological_processnervous system process
E0051932biological_processsynaptic transmission, GABAergic
E0060021biological_processroof of mouth development
E0071420biological_processcellular response to histamine
E1902476biological_processchloride transmembrane transport
E1902495cellular_componenttransmembrane transporter complex
E1902711cellular_componentGABA-A receptor complex
E1904862biological_processinhibitory synapse assembly
Functional Information from PROSITE/UniProt
site_idPS00236
Number of Residues15
DetailsNEUROTR_ION_CHANNEL Neurotransmitter-gated ion-channels signature. CmMdLrrYPlDeqnC
ChainResidueDetails
BCYS136-CYS150
CCYS151-CYS165
ACYS139-CYS153

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues233
DetailsTOPO_DOM: Extracellular => ECO:0000305
ChainResidueDetails
CGLN1-GLY234
BARG269-LYS279
EARG1-TYR220
EARG269-LYS279

site_idSWS_FT_FI2
Number of Residues88
DetailsTRANSMEM: Helical => ECO:0000305
ChainResidueDetails
CTYR235-ILE257
CALA261-ALA283
CALA295-LEU317
CTYR413-TYR435
EPHE221-ILE242
EALA246-LEU268
EALA280-VAL302
ETRP426-VAL447

site_idSWS_FT_FI3
Number of Residues94
DetailsTOPO_DOM: Cytoplasmic => ECO:0000305
ChainResidueDetails
CHIS318-SER412
BASN303-ARG425
EASN243-ASP245
EASN303-ARG425

site_idSWS_FT_FI4
Number of Residues3
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000255
ChainResidueDetails
CASN13
CASN90
CASN208
EPHE200

site_idSWS_FT_FI5
Number of Residues2
DetailsBINDING: BINDING => ECO:0000269|PubMed:24909990, ECO:0007744|PDB:4COF
ChainResidueDetails
BGLU155
EGLU155
DASN11
DASN111

site_idSWS_FT_FI6
Number of Residues6
DetailsCARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000269|PubMed:24909990
ChainResidueDetails
BASN8
BASN80
BASN149
EASN8
EASN80
EASN149

218853

PDB entries from 2024-04-24

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